论文部分内容阅读
用人抗鼠Ｐ（５３）（ＤＯ－１）单克隆抗体以ＡＢＣ免疫组织化学法检测了３８例胃癌、１１例异型增生、５例肠化生、１９例胃非癌性疾病组织及５例正常胃组织中Ｐ（５３）蛋白的表达，观察结果表明，在胃癌组织中Ｐ（５３）蛋白阳性率为５０％，异型增生中为３６％，５例肠化生中４例Ｐ（５３）蛋白呈阳性，正常胃及胃非癌性疾病组织中Ｐ（５３）蛋白均呈阴性。Ｐ（５３）蛋白阳性率与胃癌组织学类型之间看不出相关性（Ｐ＞０．０５），采用免疫胶体金标记电镜技术对胃癌细胞中Ｐ（５３）蛋白进行亚细胞定位结果，Ｐ（５３）蛋白分布于细胞核内及胞浆线粒体和粗面内质网中。Ｐ（５３）基因突变引起的Ｐ（５３）蛋白过度表达在胃癌组织中较常见，很可能在胃癌发生、发展中起重要作用。
Human anti-mouse P(53) (DO-1) monoclonal antibody was used to detect 38 cases of gastric cancer, 11 cases of dysplasia, 5 cases of intestinal metaplasia, 19 cases of gastric non-cancer disease tissue and 5 cases of normal gastric cancer by ABC immunohistochemical method. The expression of P(53) protein in stomach tissue showed that the positive rate of P(53) protein was 50% in gastric cancer tissues, 36% in dysplasia, and 4 cases of P(53) protein in 5 cases of intestinal metaplasia. Positive, normal gastric and gastric non-cancerous disease tissues were negative for P(53) protein. There was no correlation between the positive rate of P(53) protein and the histological types of gastric cancer (P>0.05). The results of subcellular localization of P(53) protein in gastric cancer cells were detected by immunogold-colloid electron microscopy. (53) Proteins are distributed in the nucleus and in cytoplasmic mitochondria and rough endoplasmic reticulum. The overexpression of P(53) protein caused by P(53) gene mutation is more common in gastric cancer tissues, and may play an important role in the occurrence and development of gastric cancer.