从美国、日本、肯尼亚等国引进8份除虫菊(Pyrethrum cinerariaefoliumTrev)材料,从中选育出优良品种"云除一号",并以其芽条为外殖体,在10种MS培养基上添加不同浓度NAA,6-BA和IBA进行了芽诱导、增殖和生根培养研究,以加速在云南产业化种植基地建设。结果表明,其效果甚为理想:MS 5培养基(MS+6-BA 1.5 mg/L+NAA 0.1 mg/L)适宜芽的诱导分化;MS 3培养基(MS+6-BA 1.5 mg/L+NAA 0.2 mg/L)适宜芽的继代增殖;MS10培养基(MS+NAA 0.3 mg/L+IBA 0.5 mg/L)适宜芽的生根。整个再生体系的建立仅需30~43 d。 Eight Pyrethrum cinerariaefoliumTrev materials were introduced from the United States, Japan, Kenya and other countries to select the best variety “Yun-one” and its bud as explants on 10 kinds of MS medium Adding different concentrations of NAA, 6-BA and IBA bud induction, proliferation and rooting studies to accelerate the construction of industrialized planting base in Yunnan. The results showed that the effect was very good: the induction of differentiation of MS 5 medium (MS + 6-BA 1.5 mg / L + NAA 0.1 mg / L) + NAA 0.2 mg / L). MS10 medium (MS + NAA 0.3 mg / L + IBA 0.5 mg / L) was suitable for rooting. The entire regeneration system was established only 30 ~ 43 d.