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目的黑木耳作为防癌抗癌的药食兼用菌被人们广泛关注,其中的多糖成分是重要的功能性成分,探索抗肿瘤作用机制对进一步开发利用黑木耳具有重要意义。方法应用逆流色谱和DEAE柱色谱方法从黑木耳中提取得到了5种不同的黑木耳多糖。结果 AAPS1、AAPS2和AAPS3均为淡黄色絮状物,AAFRB和AAFRC为白色粉末。研究了5种多糖对S180肉瘤细胞膜的活性,分析了其作用机制。研究发现,多糖AAPS2能改变肿瘤细胞膜的特性,使细胞膜脂肪酸游离;在改变细胞膜唾液酸(sialic acid,SA)含量方面,多糖可使荷瘤小鼠肿瘤细胞膜上的SA含量有明显降低(P<0.01),AAPS2活性最高;从细胞膜脂流动性分析,多糖对肿瘤细胞膜的流动性有较大影响,可显著改变细胞膜脂的流动性(P<0.05)。结论黑木耳多糖抗肿瘤作用机制可能与细胞膜上糖蛋白和糖脂链末端的残基SA及多糖促进细胞膜脂质过氧化的作用机制有关。
The purpose of black fungus as anti-cancer and anti-cancer medicine and food bacteria are widely concerned, of which polysaccharides is an important functional component, to explore anti-tumor mechanism of action for the further development and utilization of black fungus is of great significance. Methods Five different extracts of Auricularia Auricula were extracted from Auricularia auricula by countercurrent chromatography and DEAE column chromatography. Results AAPS1, AAPS2 and AAPS3 were light yellow floccules, AAFRB and AAFRC were white powder. The activity of five kinds of polysaccharides on the cell membrane of S180 sarcoma was studied, and its mechanism of action was analyzed. The study found that polysaccharide AAPS2 can change the characteristics of the tumor cell membrane, so that the cell membrane fatty acids free; in changing the cell membrane sialic acid (sialic acid, SA) content, polysaccharide can make tumor-bearing mice tumor cell membrane SA content was significantly reduced (P < 0.01), and AAPS2 had the highest activity. From the analysis of cell membrane lipid fluidity, polysaccharide had a significant effect on the fluidity of tumor cell membrane and significantly changed the fluidity of membrane lipid (P <0.05). Conclusion The anti-tumor effect of black fungus polysaccharides may be related to the action mechanism of glycoprotein and residues SA on the glycolipid chain ends and polysaccharides promoting lipid peroxidation of cell membrane.