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目的建立冠脉康片质量标准的含量测定法。方法采用Welchrom-C18(5μm,4.6×250 mm)色谱柱,以甲醇-水(30:70)为流动相,检测波长为230nm,流速1 mL.min-1。结果在此色谱条件下,超声提取15 min能较完全地提取冠脉康片中的芍药苷。芍药苷在9.5～152μg.mL-1内,峰面积与浓度呈良好的线性关系(r=0.9999),回收率均值为98.43%,RSD<2.0%。结论按上述条件,采用HPLC法测定冠脉康片芍药苷的含量,方法简便、专属强,能较好的控制冠脉康片质量。
Objective To establish a method of content determination of Guanmaikang tablet quality standard. Methods The column was Welchrom-C18 (5μm, 4.6 × 250 mm). The mobile phase was methanol-water (30:70). The detection wavelength was 230nm and the flow rate was 1 mL.min-1. Results Under this chromatographic condition, extraction of paeoniflorin in Guanmaikang tablet by ultrasonic extraction for 15 min was more complete. Paeoniflorin in 9.5 ~ 152μg.mL-1, the peak area and concentration showed a good linear relationship (r = 0.9999), the average recovery was 98.43%, RSD <2.0%. Conclusion According to the above conditions, the content of paeoniflorin in Guanmaikang tablet was determined by HPLC method. The method was simple, specific and strong, and could better control the quality of coronary capsule.