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为评价草莓枯萎病菌对多菌灵的抗性风险,从山东有代表性的草莓种植区,采集、分离了4个草莓枯萎病菌菌株,分别在含多菌灵的PDA平板培养基上进行继代培养,诱导获得抗性菌株。采用菌落生长速率法测定抗性菌株ZY-D的抗性稳定性及其对其它杀菌剂的交互抗性,比较了抗性菌株ZY-D与敏感菌株ZY的生物学特性差异。结果显示:选育至45代,抗性菌株ZY-D对多菌灵的抗性达53.91倍,抗性能够在无药条件下稳定遗传,4个抗性菌株与敏感菌株ZY具有同样的致病力;抗多菌灵菌株ZY-D对甲基硫菌灵等7种药剂表现出了不同程度的交互抗性;抗性菌株ZY-D与敏感菌株ZY的适宜生长温度均为25℃,适宜pH均为9;在不同的碳、氮源条件下,ZY-D与ZY的菌落直径和产孢量存在差异。
In order to evaluate the resistance of Fusarium oxysporum f. Sp. To carbendazim, four Fusarium oxysporum f. Sp. Isolates were collected and separated from representative strawberry growing areas in Shandong Province. Cultures were induced to obtain resistant strains. The stability of resistant strain ZY-D and its interactive resistance to other fungicides were measured by colony growth rate method. The biological characteristics of ZY-D and ZY were compared. The results showed that the resistant strain ZY-D had 53.91-fold resistance to carbendazim after 45 generations of selection, and the resistance could be stably inherited without drug. The four resistant strains showed the same resistance as the susceptible strain ZY ZY-D against seven kinds of agents such as thiophanate-methyl showed different levels of cross-resistance; the suitable growth temperature of ZY-D and ZY was 25 ℃, The optimum pH was 9. Under the different carbon and nitrogen sources, the diameters and sporulation of ZY-D and ZY were different.