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为研究荧光标记对小规格中国对虾的生长、存活率的影响以及荧光染料的保持情况,探讨VIE标记1 cm中国对虾的可行性,采用红色荧光染料标记体长为(1.14±0.06)cm的中国对虾2856尾,暂养1 d后,将其中的2321尾标记虾与6万尾同批非标记中国对虾混养于同一池塘进行了养殖试验。经112 d的饲养,结果发现,VIE标记对虾与非标记对虾在生长发育方面没有显著差异,但存活率低2.7%;VIE标记保持情况良好,虽然标记点不如制作时集中,且位置有所变化,但无论是肉眼直接观测还是用紫外光源观测都能清晰可见。通过斑尾复虾虎鱼对标记虾的捕食试验,发现标记虾不会因身体上的VIE标记而增加被斑尾复虾虎鱼捕食的机率。
In order to study the effect of fluorescent labeling on the growth and survival rate of small-sized shrimp Penaeus chinensis and the maintenance of fluorescent dyes, the feasibility of VIE labeling of 1-cm Chinese shrimp was explored. The red fluorescent dye was used to mark the length of (1.14 ± 0.06) cm At the end of 2856 prawns, after holding for 1 day, 2321 tail shrimp were incubated in the same pond with 60,000 tail-unlabeled shrimp. After 112 days of feeding, the results showed that there was no significant difference in growth and development between VIE-labeled and non-labeled shrimp, but the survival rate was 2.7% lower. The VIE marker remained in good condition. Although the marked spots were not as concentrated as the production and the positions were changed , But it can be clearly seen both in the naked eye and in the UV light source. According to the predation test of the labeled shrimp by spotted goby tiger goby, it is found that the marked shrimp will not increase the chance of predation by the spotted goby due to the body’s VIE mark.