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目的:探讨乳腺癌同源框同源物1（Six1）基因甲基化在病情及预后评估中的价值。方法:选择2015年1月至2017年1月山东省滨州市中心医院诊治的80例乳腺癌患者（乳腺癌组）和50例乳腺良性疾病患者（乳腺良性疾病组），检测两组Six1基因甲基化及mRNA表达情况，比较乳腺癌患者不同临床病理因素中Six1基因甲基化的差异。对比乳腺癌患者中Six1基因未甲基化与甲基化患者生命质量的差异。结果:乳腺癌组Six1基因未甲基化率及mRNA表达情况均高于乳腺良性疾病组，差异有统计学意义（n P<0.05）。HCC1937、MCF-7、Hs 578T及Bcap-37乳腺癌细胞中Six1基因为未甲基化状态。乳腺癌组Karnofsky评分<70分、低分化、肿瘤最大径≥ 5 cm、淋巴结转移数目≥ 9个、有远处转移及TNM分期Ⅲ + Ⅳ期患者Six1基因未甲基化率显著高于Karnofsky评分≥ 70分、高、中分化、肿瘤最大径<5 cm、淋巴结转移数目<9个、无远处转移及TNM分期Ⅰ+Ⅱ期患者[85.7%（18/21）比58.0%（40/69）、92.6%（25/27）比62.3%（33/53）、90.0%（27/30）比62.0%（31/50）、92.9%（26/28）比61.5%（32/52）、100.0%（14/14）比66.7%（44/66）、90.5%（38/42）比55.6%（20/36）]，差异有统计学意义（n P<0.05）。Six1基因未甲基化患者生命质量显著低于Six1基因甲基化患者（n P<0.05）。n 结论:Six1基因在乳腺癌中为低甲基化状态，在乳腺癌病情及预后评估中具有重要作用。“,”Objective:To detect the methylation status of sine oculis homeobox homolog 1 (Six1) in breast cancer and study its value in disease condition and prognosis evaluation.Methods:Methylation specific polymerase chain reaction (PCR) was used to detect the Six1 gene methylation in 80 patient with breast cancer (breast cancer group) and 50 patients with benign breast disease(benign breast disease group) in Binzhu Center Hospital from January 2015 to January 2017. The Six1 gene methylation rates in different clinicopathological factors were compared. Comparison of unmethylated and methylated Six1 gene in quality of life were made in breast cancer patients.Results:The unmethylation rate and mRNA expression of Six1 gene in breast cancer group were significantly higher than those in benign group (n P<0.05). Six1 genes in HCC1937, MCF-7, Hs 578T and Bcap-37 breast cancer cells were unmethylated. The unmethylated rate of Six1 gene were significantly higher in patients with Karnofsky score <70 scores, poorly differentiation, tumor maximum diameter ≥ 5 cm, number of lymph node metastases ≥ 9, with distant metastasis and TNM stage Ⅲ+Ⅳ class, compared with those in patients with Karnofsky score ≥ 70 scores, high and medium differentiation, maximum tumor diameter <5 cm, number of lymph node metastases<9, no distant metastases, and TNM stage Ⅰ+Ⅱ class. The patients with Six1 gene unmethylated had significantly lower quality of life compared with patients with Six1 gene methylated (n P<0.05).n Conclusions:Six1 gene is hypomethylated in breast cancer and plays an important role in the evaluation of breast cancer disease condition and prognosis.