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基因治疗的效果严重依赖于基因载体。与传统包封技术相比,在自组装技术基础上发展起来的以DNA为聚阴离子,与荷正电的高分子材料在溶液中形成纳米粒的方法,已成为目前最重要的非病毒基因载体制备手段,具有良好的应用前景。采用层层自组装(layer-by-layer assembly,LbL)技术可提高基因装载率,其优势还在于纳米粒表面性质的可控性:在温和的条件下实现多种材料在载体表面的固定,实现载体多功能化等。本文将对近年来国内外有关层层自组装纳米粒作为非病毒基因载体的研究进展以及本课题组在此方向的研究进行简要综述。
The effect of gene therapy depends heavily on the gene vector. Compared with the traditional encapsulation technology, the self-assembly technology developed on the basis of DNA as a polyanion, and positively charged polymer materials in solution to form nanoparticles has become the most important non-viral gene carrier Preparation method, has a good prospect. Adopting layer-by-layer assembly (LbL) technology can increase the gene loading rate, and its advantage lies in the controllability of the surface properties of the nanoparticles: in a mild condition to achieve a variety of materials in the carrier surface fixation, To achieve multi-carrier and so on. In this paper, we will review the research progress of self-assembled nanoparticles at different levels both at home and abroad as non-viral gene carriers and the research of our group in this direction.