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目的:研究HDM通过肺泡巨噬细胞(AM)Toll样受体4(TLR4)的高表达及诱导AM的活化,探讨其对哮喘小鼠气道炎症的影响。方法:BALB/c小鼠随机分为哮喘模型组(OVA)A,HDM处理组(HDM+OVA)B,对照组(生理盐水)C。用卵白蛋白(OVA)致敏与激发建立小鼠哮喘模型;HE染色观察小鼠气道及肺组织病理变化;光学显微镜下观察小鼠支气管肺泡灌洗液(BALF)中细胞分类及计数;酶联免疫吸附试验(ELISA)检测小鼠BALF上清中IL-4、IL-5、IL-13和IFN-γ的含量,实时定量PCR法测定AM的TLR4的表达,流式细胞技术(FCM)检测AM的CD80、CD86的表达。结果:与A组相比,B组BALF上清中IL-4、IL-5和IL-13的水平显著增高(P<0.05),而IFN-γ差异无统计学意义(P>0.05),与A组相比,AM的TLR4mRNA表达明显增高(P<0.05),CD80的表达差异无统计学意义,CD86的表达水平显著增高,差异有统计学意义(P<0.05)。结论:HDM通过AM的TLR4的高表达诱导AM的活化,加重哮喘的气道炎症。
OBJECTIVE: To study the effect of HDM on the expression of TLR4 and the activation of AM in alveolar macrophages (AM), and to explore its effect on airway inflammation in asthmatic mice. Methods: BALB / c mice were randomly divided into asthma model group (OVA) A, HDM treatment group (HDM + OVA) B, control group (saline) Mouse asthma model was induced by ovalbumin (OVA) sensitization and challenge; the pathological changes of airway and lung were observed by HE staining; the cell sorting and counting in bronchoalveolar lavage fluid (BALF) were observed under light microscope; The levels of IL-4, IL-5, IL-13 and IFN-γ in BALF supernatants of mice were detected by ELISA. The expression of TLR4 in AM was detected by real-time quantitative PCR. Flow cytometry (FCM) Detection of AM CD80, CD86 expression. Results: Compared with group A, the levels of IL-4, IL-5 and IL-13 in BALF supernatants of group B were significantly increased (P <0.05), but there was no significant difference of IFN- Compared with group A, the expression of TLR4 mRNA in AM was significantly increased (P <0.05). The expression of CD80 was not significantly different, but the expression of CD86 was significantly increased. The difference was statistically significant (P <0.05). CONCLUSIONS: HDM induces AM activation through AM TLR4 overexpression, exacerbating airway inflammation in asthma.