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目的建立测定舒胆片中大黄素、大黄酚的含量的HPLC法。方法以C1 8柱为填充剂,以甲醇-水-磷酸(85:15:0.01)为流动相;检测波长:254 nm。进样体积:10μL。结果大黄素进样量在0.0662~0.662μg范围内与峰面积呈良好的线性关系。平均回收率大黄素100.42%RSD为2.4;大黄酚在0.0685~0.685μg范围内与峰面积呈良好的线性关系。平均回收率为99.21%,RSD为2.7%。结论此法简便、准确、重现性好,可用于舒胆片中大黄素、大黄酚的含量测定和质量控制
Objective To establish an HPLC method for the determination of emodin and chrysophanol in Shu Dan Tablets. Methods The mobile phase consisted of C1 8 column and methanol - water - phosphoric acid (85: 15: 0.01) as the mobile phase. The detection wavelength was 254 nm. Injection volume: 10μL. Results Emodin injection volume in the range of 0.0662 ~ 0.662μg showed a good linear relationship with the peak area. The average recovery was 100.42% RSD of emodin was 2.4. Chrysophanol showed a good linear relationship with the peak area in the range of 0.0685 ~ 0.685μg. The average recovery was 99.21% with a RSD of 2.7%. Conclusion This method is simple, accurate, reproducible, and can be used for the content determination and quality control of emodin and chrysophanol