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比较流动培养的动脉、静脉和微血管来源内皮细胞(EC)黏附性能有无差异,探索它们在相似的流动培养条件下能否获得与移植物相当的黏附强度。采用流动培养系统,通过精密蠕动泵提供剪切力,同时提供静态培养所需其他条件,建立体外EC流动培养模型,比较三种来源途径的静态培养EC与动态培养EC在相同剪切力作用下的黏附情况,并研究EC骨架成分肌动蛋白丝的分布及在剪切力作用下的变化。流动培养后EC均沿流动方向排列并延长;静态培养EC的黏附强度以动脉EC最大,微血管EC其次,静脉EC明显低于前两者(P值均<0.05);各种来源EC动态培养后黏附强度均较相应静态培养有显著提高,动脉EC与微血管EC的细胞残留率差异无统计学意义,而静脉EC明显低于前两者(P值均<0.05)。流动培养后肌动蛋白丝均聚集成束,沿剪切力方向有序排列,致密周围带逐渐消失,形成明显的张力纤维,在微血管EC更相互联系形成一个整体。在相似的流动培养条件下,三种不同来源的EC的黏附强度较静态培养大幅度提高,且微血管来源EC能够达到与动脉EC相当的黏附强度。
To compare the adhesion properties of arterial, venous and microvascular derived endothelial cells (ECs) between mobile cultures, and to find out whether they can achieve the same adhesion strength as that of implants under similar flow culture conditions. The flow culture system was used to provide the shear force through the precision peristaltic pump. At the same time, other conditions needed for static culture were provided. The EC flow culture model in vitro was established. The static culture EC and dynamic culture EC were compared under the same shear force Of the adhesion situation, and to study the distribution of the EC framework actin filaments and shear changes in the role. The ECs of the cultured ECs were the largest in the arterial ECs, followed by the ECs of the microvessels ECs and the ECs in the vein were significantly lower than those in the former two groups (all P <0.05). After EC culture of various sources The adhesion strength was significantly higher than that of the corresponding static culture. There was no significant difference in the cell residual rate between arterial EC and microvascular EC, but the venous EC was significantly lower than the former two groups (P <0.05). Actin filaments were aggregated into bundles after flow culture, and arranged orderly along the direction of shear force. The bands around the dense gradually disappeared, forming obvious tension fibers, which formed a whole in the microvascular ECs. Under similar conditions of flow culture, the adhesion strength of EC from three different sources increased significantly compared with that of static culture, and the microvascular derived EC could reach the adhesion strength comparable with arterial EC.