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目的探讨地黄对去卵巢骨质疏松大鼠股骨骨密度、腰椎骨组织整合素β1 mRNA表达的影响。方法 72只雌性大鼠,随机分为假手术组、模型组、雌二醇组和地黄小、中、大剂量组。假手术组仅行假手术,其余五组行卵巢切除术。术后1w分别灌胃给予17β-雌二醇和地黄小、中、大剂量,连续给药3个月。测定血钙、磷和碱性磷酸酶(ALP),尿钙(u-Ca)、磷、尿脱氧吡啶酚(D-Pyr)和肌酐(Cr)。之后处死动物,取出右侧股骨,测定骨密度;取出第2腰椎,测定骨钙(b-Ca)、磷(b-P)含量;取出第4腰椎,采用实时荧光逆转录聚合酶链反应测定腰椎骨整合素β1 mRNA表达。结果与假手术组相比,模型组血清ALP、u-Ca、D-Pyr/Cr显著增加,股骨密度、腰椎骨整合素β1 mRNA表达均降低。与模型组比较,地黄中、大剂量组和雌二醇组均可使血清ALP、u-Ca、D-Pyr/Cr排出量降低,股骨密度、腰椎骨整合素β1 m RNA表达均增加。结论地黄能抑制由于去卵巢雌激素缺乏引发的骨转换增强,提高骨密度,促进腰椎骨组织整合素β1 m RNA表达,增强骨质量。
Objective To investigate the effects of Rehmanniae on femoral bone mineral density (BMD) and integrin β1 mRNA expression in lumbar vertebra in ovariectomized rats. Methods Seventy-two female rats were randomly divided into sham operation group, model group, estradiol group, and Rehmannia glutinosa small, medium and large dose groups. Sham-operated group only sham surgery, the remaining five groups underwent ovariectomy. After 1w, 17β-estradiol and rehmanniae were administered intragastrically to small, medium and large doses, respectively, for continuous administration for 3 months. Blood calcium, phosphorus and alkaline phosphatase (ALP), urinary calcium (u-Ca), phosphorus, D-Pyr and creatinine were measured. The animals were sacrificed, the right femur was removed and the bone density was measured. The second lumbar vertebra was removed and the contents of b-Ca and bP were measured. The fourth lumbar vertebra was removed and the lumbar vertebrae were measured by real-time fluorescence reverse transcription polymerase chain reaction Integrin β1 mRNA expression. Results Compared with the sham operation group, the levels of ALP, u-Ca and D-Pyr / Cr in the model group were significantly increased, while the femoral density and lumbar integrβ1 mRNA expression were decreased. Compared with the model group, the excretion of ALP, u-Ca, D-Pyr / Cr and the density of femur and the expression of integrin β1 mRNA in lumbar vertebrae both increased in medium and large doses of Rehmannia glutinosa group and in estradiol group. Conclusion Rehmanniae can inhibit the increase of bone turnover induced by ovariectomized estrogen deficiency, increase the bone mineral density, promote the expression of integrin β1 m RNA in lumbar vertebra and enhance the bone quality.