[目的]通过对骨髓石蜡包埋组织中脱钙方法的探讨,旨在改进骨髓穿刺组织活检石蜡切片的质量。[方法]骨髓组织先行石蜡包埋,再脱钙,最后染色。[结果]切片完整无缺,组织结构严密,细胞形态良好,染色清晰。[结论]脱钙方法简单、可靠和实用,适合普及推广。 [Objective] To discuss the method of decalcification in bone marrow paraffin embedded tissue and aim to improve the quality of biopsy paraffin sections of bone marrow aspiration tissue. [Methods] Bone marrow was paraffin-embedded, decalcified and finally stained. [Result] The section was intact, the structure was tight, the cell morphology was good and the staining was clear. [Conclusion] The decalcification method is simple, reliable and practical, suitable for universal popularization.