我们在松材线虫永久玻片制作中,对常用方法作了一些修改,现介绍供试用。一、材料选择从松木及其制品中分离得到的线虫,经临时玻片观察存在大量线虫成虫,可直接制作永久玻片。二、制作程序分杀死、固定、脱水透明和封片四个步骤。1.杀死将分离的线虫液5ml置于离心管中,加热(52℃,5分钟)杀死虫体,离 We made some changes to the commonly used methods in the production of permanent slides for pine wood nematodes and are now available for trial use. First, the material selection Pine and its products from the isolated nematodes, the temporary slide observation of large numbers of adult worms, can be directly produced permanent glass. Second, the production process points to kill, fixed, dehydrated and sealed four steps transparent. 1. Kill the isolated nematode fluid 5ml placed in a centrifuge tube, heated (52 ℃, 5 minutes) to kill parasites, from