采用本实验室新建的人肝癌细胞系GHC-1及GHC-3分泌的AFP作抗原,经微量法免疫BALB/c小鼠,通过鼠-鼠途径,获得两株具有较高专一性的分泌抗人肝癌AFP-McAb杂交瘤细胞,定名为GAH_1及GAH_2。该两株杂交瘤细胞分泌的McAb分别为IgG_3及IgG_2b亚型。用ELISA-Ⅰ、DIBA和 RPHA-Ⅰ筛检、鉴定结果,其中GAH_1和GAH_2分泌的McAb可抑制肝癌患者血清和腹水,对胎血呈不完全抑制,而对其他各种肿瘤病人均为阴性,表示其对人肝癌具有较高的专一性。 本文通过对抗原来源的选择和筛检方法的改进,取得较为满意的效果。 BALB / c mice were immunized with the AFP secreted by human hepatocellular carcinoma cell lines GHC-1 and GHC-3 newly established in our laboratory. BALB / c mice were immunized by the mouse-mouse route to obtain two secretions with higher specificity Anti-human liver cancer AFP-McAb hybridoma cells, named GAH_1 and GAH_2. The McAbs secreted by the two hybridoma cells were IgG3 and IgG2b subtypes, respectively. The results of ELISA-Ⅰ, DIBA and RPHA-Ⅰ screening showed that the McAbs secreted by GAH_1 and GAH_2 could inhibit the serum and ascites of patients with liver cancer, but did not completely inhibit the fetal blood, but negative for other kinds of cancer patients. That its high specificity for human liver cancer. In this paper, through the selection of antigen sources and screening methods to improve, get more satisfactory results.