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目的:探讨当归多糖(APS)对调控红系血细胞增殖分化有关的细胞内JAK2/STAT5信号传导通路的影响,阐述当归多糖促进血细胞发生的可能机制。方法:分离纯化胎儿脐带血单个核细胞,在常规体外培养体系加入APS(200 mg.L-1)作用细胞24 h,促红细胞生成素(Epo)分别刺激0,2,5,30 m in,免疫细胞化学与激光共聚焦显微镜观察细胞STAT5的表达;W estern-b lotting增强化学发光法检测细胞JAK2与浆和核中STAT5的表达。结果:APS协同Epo对STAT5的表达影响显著,对照组与APS组在4个时间点STAT5的表达水平有显著差异,JAK2、细胞浆和核中STAT5表达较对照组显著增加,且在Epo刺激5 m in时表达最强。结论:APS能协同Epo促进造血细胞增殖分化,其机制与影响细胞内的JAK2/STAT5信号传导通路有关。
Objective: To investigate the effect of polysaccharide from Angelica sinensis (APS) on the regulation of JAK2 / STAT5 signal transduction pathway involved in the proliferation and differentiation of erythrocytic blood cells, and to elucidate the possible mechanism of Angelica polysaccharide in promoting hematopoiesis. Methods: Cord blood mononuclear cells were isolated and purified from fetal umbilical cord blood mononuclear cells (PBMCs). The cells were treated with APS (200 mg.L-1) for 24 h in vitro and erythropoietin (Epo) Immunocytochemistry and laser confocal microscopy were used to observe the expression of STAT5 in cells; Western-b lotting enhanced chemiluminescence was used to detect the expression of JAK2 and STAT5 in plasma and nucleus. Results: The expression of STAT5 was significantly affected by APS in combination with Epo. The expression of STAT5 in control group and APS group was significantly different at 4 time points. The expression of STAT5 in cytoplasm and nucleus of JAK2 increased significantly compared with control group m in the expression of the strongest. Conclusion: APS can synergize with Epo to promote the proliferation and differentiation of hematopoietic cells. Its mechanism is related to the influence of JAK2 / STAT5 signal transduction pathway in cells.