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为探讨丙型肝炎（ＨＣ）病人细胞免疫功能和丙型肝炎病毒（ＨＣＶ）的致病机制及机体对其免疫保护作用，收集２４例ＨＣ病人（急性３例，慢性２１例），用３Ｈ－ＴｄＲ掺入法研究病人外周血单个核细胞（ＰＢＭＣ）对不同ＨＣＶ抗原增殖反应，并用流式细胞仪（ＦＡＣＳ）检测了ＰＢＭＣ中ＣＤ４＋、ＣＤ８＋淋巴细胞亚群在ＨＣＶ抗原刺激后的变化。结果：ＨＣ病人ＰＢＭＣ对ＨＣＶ合成肽ＣＰ９，ＮＳ４和基因重组抗原Ｃ，Ｅ１，Ｅ２，ＮＳ３刺激后出现不同程度增殖反应，刺激指数（ＳＩ）分别为１．６９±０．５１，１．６１±０．５４，１．６８±０．５８，１．４９±０．４４，１．４４±０．４４和１．３３±０．３３。３例急性ＨＣ中２例病人的ＰＢＭＣ对ＨＣＶ抗原呈有效增殖反应（ＳＩ≥２．１），且血清ＨＣＶＲＮＡ阴转伴ＡＬＴ正常。细胞表型分析显示：增殖的细胞表型是ＣＤ４＋淋巴细胞，而ＣＤ８＋淋巴细胞增殖反应较弱。结论：ＨＣ病人ＰＢＭＣ确实存在对ＨＣＶ抗原的增殖反应；ＣＤ４＋淋巴细胞比ＣＤ８＋淋巴细胞增殖反应要强，急性ＨＣ病人ＰＢＭＣ对ＨＣＶ抗原有效的增殖反应预示可能有良好的临床愈合
To investigate the cellular immune function and the pathogenic mechanism of hepatitis C virus (HCV) in hepatitis C (HC) patients and to protect their immune system, 24 HC patients (3 acute and 21 chronic) were collected, TdR incorporation method was used to study the proliferative response of PBMCs to different HCV antigens. The changes of CD4 +, CD8 + lymphocyte subsets in PBMC after stimulation with HCV antigen were detected by flow cytometry (FACS). Results: PBMC of HC patients showed proliferative responses to various degrees after stimulated with HCV synthetic peptides CP9, NS4 and recombinant antigens C, E1, E2 and NS3 with the SI values of 1.69 ± 0.51 and 1.61 ± 1.71 0.54, 1.68 ± 0.58, 1.49 ± 0.44, 1.44 ± 0.44 and 1.33 ± 0.33 PBMC of 2 patients with acute HC Effective proliferative response (SI ≥ 2.1), and serum HCVRNA negative with ALT normal. Cell phenotype analysis showed that: the proliferating cell phenotype is CD4 + lymphocytes, and CD8 + lymphocyte proliferation response is weak. CONCLUSIONS: The PBMCs of HC patients do have a proliferative response to HCV antigens. CD4 + lymphocytes are more proliferative than CD8 + lymphocytes. The effective proliferative response of PBMC to HCV antigens in acute HC patients indicates that there may be good clinical healing