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目的:探讨电针肝俞和肾俞对大脑中动脉梗塞(MCAO)模型大鼠脑梗死灶周围血管新生相关因子血管内皮生长因子(VEGF)、血小板-内皮细胞粘附分子(PECAM-1)/CD31的影响及可能机制,为针刺治疗脑缺血中风提供新方案。方法:将180只健康雄性Sprague-Dawley(SD)大鼠随机分为假手术组、模型组、穴位组和非穴组,每组45只。除假手术组外,其余各组大鼠均采用改良线栓法制备MCAO模型;穴位组予电针肝俞和肾俞治疗,非穴组予电针非穴点治疗,其余两组大鼠只捆绑,不治疗。在MCAO术后针灸刺激的第3 d、14 d及21 d三个时间点各组随机抽10只大鼠测试大鼠神经缺损症状;同时检测CD31和VEGF的表达量。结果:与模型组和非穴组比较,穴位组各时相的神经功能评分降低,组间差异具有统计学意义(P<0.05,P<0.01)。各组大鼠VEGF和CD31的表达在第3 d时最低,于14 d达高峰,第21 d仍维持在较高水平,各组第14 d与第21 d比较均有统计学意义(P<0.05,P<0.01)。与模型组及非穴组比较,穴位组各时相VEGF和CD31的表达升高,组间差异具有统计学意义(均P<0.05)。结论:电针肝俞和肾俞能明显改善MCAO模型大鼠神经功能评分,对脑缺血有保护作用,保护机制可能与电针上调MCAO模型大鼠梗死灶周围CD31和VEGF表达,诱导血管新生有关。
Objective: To investigate the effects of electroacupuncture at “Ganshu” and “Shenshu” on the expression of vascular endothelial growth factor (VEGF), platelet-endothelial cell adhesion molecule (PECAM-1) and vascular endothelial cell adhesion molecules (VEGF) in the peripheral blood of cerebral infarction rats with middle cerebral artery occlusion CD31 and its possible mechanism to provide a new protocol for acupuncture treatment of cerebral ischemic stroke. Methods: 180 healthy male Sprague-Dawley (SD) rats were randomly divided into sham operation group, model group, acupoint group and non-acupoint group, 45 rats in each group. In addition to the sham-operation group, the other rats in each group were treated by modified suture method to prepare MCAO model. The acupoints were treated with electro-acupuncture at liver-shu and Shenshu. The non-acupoint group was treated by electro-acupuncture at non-acupoints. Bundled, not treated. At 3, 14 and 21 d after acupuncture stimulation in MCAO, 10 rats in each group were randomly selected to test the neurological deficit symptoms. The expressions of CD31 and VEGF were detected simultaneously. Results: Compared with the model group and the non-acupoint group, the score of neurological function in acupoint group was decreased at each time point, the difference between the two groups was statistically significant (P <0.05, P <0.01). The expression of VEGF and CD31 in each group was the lowest on the 3rd day, reaching the peak on the 14th day, remained high on the 21st day, and the difference was statistically significant on the 14th and 21st day in each group (P < 0.05, P <0.01). Compared with the model group and the non-acupoint group, the expression of VEGF and CD31 increased at each time point in acupoint group, with significant difference between the two groups (all P <0.05). CONCLUSION: Electroacupuncture of liver and kidney can significantly improve the neurological function score of MCAO rats, which has a protective effect on cerebral ischemia. The protective mechanism may be related to that EA increases the expression of CD31 and VEGF in the infarct zone of MCAO rats and induces angiogenesis related.