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目的探讨PPAR-γ在脑膜瘤中的基因和蛋白表达以及其激动剂可能的抗肿瘤作用。方法应用RT-PCR、免疫组化方法对48例脑膜瘤的手术标本进行PPAR-γ的基因和蛋白表达分析,并体外培养16例人脑膜瘤细胞,应用MTT方法探讨曲格列酮对脑膜瘤细胞生长的作用,应用流式细胞仪检测曲格列酮干预后凋亡情况。结果PPAR-γ在48例脑膜瘤细胞中均有表达,且非典型组高于良性组(P<0.05),女性患者PPAR-γ基因表达高于男性;其表达与脑膜瘤增殖性正相关,曲格列酮体外抑制脑膜瘤细胞生长,并可以诱导脑膜瘤细胞凋亡,其作用具有时间和浓度的依赖性。结论非典型脑膜瘤PPAR-γ表达高于良性组,其表达与增殖活性成正相关;其配体活化后体外可以抑制脑膜瘤细胞生长并可诱导凋亡。
Objective To investigate the gene and protein expression of PPAR-γ in meningioma and the possible anti-tumor effect of its agonist. Methods The gene and protein expression of PPAR-γ in 48 cases of meningiomas were analyzed by RT-PCR and immunohistochemistry. 16 human meningioma cells were cultured in vitro. MTT was used to detect the expression of PPAR-γ in meningiomas The effect of troglitazone on apoptosis was detected by flow cytometry. Results PPAR-γ was expressed in 48 cases of meningioma cells, and atypical group was higher than that in benign group (P <0.05). The expression of PPAR-γ gene in female patients was higher than that in male patients. The expression of PPAR-γ was positively correlated with the proliferation of meningiomas, Troglitazone inhibits the growth of meningioma cells in vitro and induces meningioma cell apoptosis in a time and concentration dependent manner. Conclusions The expression of PPAR-γ in atypical meningioma is higher than that in benign meningioma. The expression of PPAR-γ is positively correlated with the proliferation of meningioma. The activation of its ligand can inhibit meningioma cell growth and induce apoptosis in vitro.