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以先玉335为母本、高频孤雌生殖诱导系G5为父本杂交诱导产生的单倍体为实验材料,研究秋水仙素浸芽加倍处理玉米单倍体的最佳浓度和时间。结果表明,秋水仙素浓度为0.7 mg/m L、浸芽8 h处理下的玉米单倍体加倍率最高,高达14.4%;秋水仙素浓度为0.8 mg/m L、浸芽8 h处理下的单倍体加倍率次之,为12.1%。以自交系PHB1M、PH4CV、PH6WC、PHGJ4和DH系Z2、Z8为亲本组配的PHB1M×PH4CV、PHB1M×Z2、PH4CV×DZ2、PH6WC×PHGJ4、PH6WC×Z8、PHGJ4×Z8组合为母本,以G5为父本,研究玉米DH系对杂交后代加倍率的影响,结果表明,PH4CV×Z2(26.2%)、PHB1M×Z2(25.8%)、PHGJ4×Z8(23.3%)和PH6WC×Z8(21.3%)的单倍体加倍率显著高于PHB1M×PH4CV(13.5%)和PH6WC×PHGJ4(8.4%),以DH系为亲本组配选系基础材料可显著提高玉米杂交组合的单倍体加倍率。
Hyphenated maize haploid was treated with Xianjian 335 as female parent and high frequency parthenogenetic induction line G5 as male parent to produce haploid. The results showed that when the concentration of colchicine was 0.7 mg / m L, the doubling rate of maize haploid was the highest (14.4%) at 8 h after bud soaking, the concentration of colchicine was 0.8 mg / m L, Haploid doubling rate, followed by 12.1%. PHB1M × PH4CV, PHB1M × Z2, PH4CV × DZ2, PH6WC × PHGJ4, PH6WC × Z8 and PHGJ4 × Z8 with the inbred lines PHB1M, PH4CV, PH6WC, PHGJ4 and DH lines Z2 and Z8 as the parent , The effects of maize DH line on the doubling rate of hybrid progeny were studied using G5 as the male parent. The results showed that the ratio of PH4CV × Z2 (26.2%), PHB1M × Z2 (25.8%), PHGJ4 × Z8 (23.3%) and PH6WC × Z8 21.3%) was significantly higher than that of PHB1M × PH4CV (13.5%) and PH6WC × PHGJ4 (8.4%). The haplotypes based on DH lines could increase the haploid population Double rate.