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以本室构建的双价痢疾候选疫苗株ＦＳ５４１６ 作为口服免疫原，检测了小鼠ＰＰ 结（Ｐｅｙｅｒ ，ｓ ｐａｔｃｈｅｓ） 中Ｔ 淋巴细胞的体外增殖反应， 观察了腹腔注射抗Ｌ３Ｔ４ ｍ Ａｂ 对小鼠肠粘膜部位产生特异性Ｉｇ 分泌细胞的影响。结果表明：①小鼠口服免疫后，ＰＰ 结中的ＣＤ４ ＋ Ｔ 细胞可产生明显的特异性增殖反应：②选择性地删除ＣＤ４ ＋ Ｔ 细胞后，小鼠肠粘膜无抗原特异性Ｉｇ 分泌细胞产生。以上提示，小鼠肠粘膜对痢疾疫苗的免疫应答需要ＣＤ４ ＋ Ｔ 细胞的参与。
In this study, the bivalent dysentery vaccine strain FS5416 was used as an oral immunogen to detect the in vitro proliferative response of T lymphocytes in mice PP (Peyer, s patches). The effects of intraperitoneal injection of anti-L3T4 m Ab on small Effect of Specific Ig Secreted Cells in Rat Mucosal Mucosa. The results showed that: (1) CD4 + T cells in PP knots could produce specific and specific proliferative responses after mice were orally immunized: (2) After selective deletion of CD4 + T cells, there was no antigen-specific Ig secreting cells in mouse intestinal mucosa . The above suggests that mouse intestinal mucosa immune response to diarrhea vaccine requires the involvement of CD4 + T cells.