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目的:观察中药丹参多酚酸盐(salvianolate)对C57BL/6J ApoE~(-/-)小鼠血清白细胞介素-6(Interleukin-6,IL-6)的影响,以探讨其抗动脉粥样硬化的可能机制。方法:给4周龄雄性C57BL/6J ApoE~(-/-)小鼠喂食高脂饮食,腹腔注射丹参多酚酸盐,随机将其分为模型组(仅腹腔注射生理盐水)、丹参多酚酸盐低剂量(60mg/kg)组、中剂量(120mg/kg)组、高剂量(240mg/kg)组,每组6只,共24只;正常对照组(即C57BL/6J野生型小鼠)5只。24周末时处死各组小鼠,留取血清,采用特异性放射免疫分析各组小鼠血清IL-6水平。结果:24周末,随丹参多酚酸盐剂量的增加,ApoE~(-/-)小鼠血清浓度逐渐降低,丹参多酚酸盐各浓度组血清IL-6水平均明显低于模型组(P均<0.05);丹参多酚酸盐120mg/kg组血清IL-6水平明显低于60mg/kg组(P<0.05);丹参多酚酸盐240mg/kg组血清IL-6水平明显低于120mg/kg组和60mg/kg组(P<0.05),丹参多酚酸盐240mg/kg组IL-6浓度仍明显高于正常对照组水平。结论:丹参多酚酸盐各剂量组均能够降低ApoE~(-/-)小鼠血清IL-6水平,随剂量增加,减低越明显,说明丹参多酚酸盐能够抑制动脉粥样硬化炎症反应,可能是其抗动脉粥样硬化的作用机制之一。
OBJECTIVE: To observe the effect of Salvianolate on serum Interleukin-6 (IL-6) in C57BL / 6J ApoE ~ (- / -) mice to explore its anti-atherosclerotic Possible mechanism of hardening. Methods: A 4-week-old male C57BL / 6J ApoE ~ (- / -) mice were fed a high-fat diet and intraperitoneal salvianolate injection. The rats were randomly divided into model group In the control group (C57BL / 6J wild-type mice, the low dose of 60mg / kg, the middle dose of 120mg / kg and the high dose of 240mg / ) 5 only. At the end of the 24th week, mice in each group were killed and serum was collected. Serum IL-6 level in each group was detected by radioimmunoassay. Results: At the end of the 24th week, serum concentration of ApoE ~ (- / -) mice gradually decreased with the increase of dose of Salvia miltiorrhiza bunge and the level of IL-6 in Salvia miltiorrhiza Bunge was lower than that of model group (P <0.05). The serum level of IL-6 in Salviae miltiorrhizae polyphenols 120mg / kg group was significantly lower than that in 60mg / kg group (P <0.05) / kg group and 60mg / kg group (P <0.05). The concentration of IL-6 in salvianolate 240mg / kg group was still significantly higher than that of the normal control group. Conclusion: Salviae miltiorrhizae polyphenols can reduce serum IL-6 level in ApoE ~ (- / -) mice with increasing dose, and the more obvious the decrease is, indicating that Salviae miltiorrhiza polyphenols can inhibit atherosclerosis inflammatory response , May be one of its anti-atherosclerosis mechanism.