目的:采用低温超声波提取贯叶金丝桃素,建立贯叶金丝桃中贯叶金丝桃素含量测定方法。方法:用高效液相色谱法测定。色谱柱:十八烷基硅烷键合硅胶柱,流动相:0.1%磷酸—乙腈(10∶90),流速(1.0 mL/min),检测波长210 nm,柱温:25℃。结果:线性范围:1.8930~4.4170μg。稳定性:RSD=0.70%,n=5。精密度:RSD=1.06%,n=6。重复性:RSD=0.87%,n=5。回收率:99.13%,RSD=1.20%,n=9。符合有关规定,其他方法学考查均符合有关规定。结论:此方法专属性强、重复性好,可用于贯叶金丝桃原料及提取物中贯叶金丝桃素含量测定。 OBJECTIVE: To establish a method for the determination of hyperforin in Hypericum perforatum by extraction of Hypericin from Hyperhual. Methods: Determination by high performance liquid chromatography. Column: octadecylsilane bonded silica gel, mobile phase: 0.1% phosphoric acid-acetonitrile (10:90), flow rate 1.0 mL / min, detection wavelength 210 nm, column temperature 25 ℃. Results: The linear range: 1.8930 ~ 4.4170μg. Stability: RSD = 0.70%, n = 5. Precision: RSD = 1.06%, n = 6. Repeatability: RSD = 0.87%, n = 5. Recovery: 99.13%, RSD = 1.20%, n = 9. In line with the relevant provisions, other methodological tests are in line with the relevant provisions. Conclusion: This method is highly specific and reproducible. It can be used for the determination of Hyperforum in Hypericum perforatum and its extracts.