[目的]建立一种高效液相色谱法同时测定莠灭净和敌草隆的定量分析方法。选用甲醇-水作流动相,RP-18色谱柱,检测波长254 nm,外标法定量。[结果]方法的回收率为99.0%~101.0%,标准偏差0.180~0.192,变异系数1.75%~1.79%。[结论]该方法简便、快速、能进行准确定量分析,结果稳定。 [Objective] The research aimed to establish a method for simultaneous determination of ametryn and diuron by high performance liquid chromatography. The mobile phase of methanol-water was selected as RP-18, the detection wavelength was 254 nm and the external standard method was used. [Result] The recovery rate was 99.0% ~ 101.0%, the standard deviation was 0.180 ~ 0.192, the coefficient of variation was 1.75% ~ 1.79%. [Conclusion] The method is simple, rapid, accurate quantitative analysis, the results are stable.