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目的 通过腺苷 (ADO)改善卡介苗 (BCG)初次免疫接种效果 ,提高巨噬细胞抗结核杆菌毒力株效能。方法 将BALB/C小鼠随机分BCG组、ADO组和对照组。对BCG、ADO两组分别一次性皮内注射卡介苗 0 1ml。对ADO组腹腔注射ADO 30mg/(kg·d) ,对BCG组、对照组腹腔注射生理盐水 0 1ml/d ,共 5d。 6周后 ,三组均经血管接种人型结核分枝杆菌标准毒力株 1× 10 6CFU ,BCG组、ADO组于结核杆菌感染后第 10、2 0天和 30天分批取材 ,对照组于第 30天取材。将肺、脾用多聚甲醛固定 ,石蜡包埋 ,HE染色与抗酸染色 ,进行原位细胞凋亡检测。结果 对照组结核杆菌感染后第30天 ,肺组织大部分实变 ,以中性粒细胞浸润为主 ,结核结节多 ,肺实变与肺泡隔重度增宽处弥漫分布大量菌体 ;BCG组于感染后第 10～ 30天肺泡隔增宽 ,逐渐加重至肺大部分实变 ,间质内以淋巴细胞浸润为主 ,结核结节较少 ,肺内大量菌体 ;而ADO组肺实变和肺泡隔重度增宽的面积较小 ,间质内以单核细胞浸润为主 ,结核结节很少 ,肺内菌体明显减少。结论 ADO促进BCG诱导的免疫记忆性反应中能引起单核 巨噬细胞的数量增多和杀菌能力的增强
Objective To improve the initial immunization effect of BCG by adenosine (ADO) and enhance the potency of antimycobacterium tuberculosis strains. Methods BALB / C mice were randomly divided into BCG group, ADO group and control group. BCG, ADO two groups were once intradermally injected BCG 0 1ml. The rats in ADO group were injected intraperitoneally with ADO 30mg / (kg · d), while the BCG group and control group were intraperitoneally injected with saline 0.1ml / d for 5 days. After 6 weeks, all the three groups were inoculated with 1 × 10 6 CFU of standard virulent strain of Mycobacterium tuberculosis, and BCG group and ADO group were obtained in batches on the 10th, 20th and 30th day after the infection with Mycobacterium tuberculosis. The control group Draw on the 30th day. The lungs and spleen were fixed with paraformaldehyde, embedded in paraffin, HE staining and acid-fast staining were used to detect apoptosis in situ. Results In the control group, on the 30th day after infection with Mycobacterium tuberculosis, most of the lung tissues were solidified, with mainly neutrophil infiltration, more nodules of tuberculosis, more pulmonary consolidation and more severe alveolar septum diffusion. Alveolar septum widened on the 10th to 30th day after infection and gradually increased to most of the consolidation of the lungs. The main infiltration of lymphocytes in the interstitium, less tuberculous nodules, a large number of bacteria in the lung; ADO group of lung consolidation And alveolar septum widened the area of smaller, interstitial mononuclear cell infiltration, tuberculous nodules, pulmonary cells significantly reduced. CONCLUSION ADO can promote the increase of the number of monocyte-macrophage and the enhancement of bactericidal activity induced by BCG in immune-memory reaction