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采用TLC法定性鉴别复方丹参胶囊中的三七和丹参,并以HPLC法测定了其中水溶性成分丹酚酸B。鉴别三七时,样品先上固相萃取小柱用水和30%甲醇洗涤,以减少杂质对检测的干扰,再用甲醇洗脱,洗脱液用二氯甲烷-无水乙醇-水(70:45:6.5)为展开剂进行TLC,结果可使三七中的三七皂苷R1,人参皂苷Re、Rb1和Rg1达到有效分离。测定丹酚酸B含量时,采用C18柱,以甲醇-乙腈-甲酸-水(30:10:1:59)为流动相,检测波长为286 nm。丹酚酸B在0.1～10 g范围内线性关系良好。本研究所建立的定性和定量方法简便、准确、专属性强,可有效控制复方丹参胶囊的质量。
The TLC method was used to identify Panax notoginseng and Salvia miltiorrhiza in Compound Danshen capsule. The water-soluble salvianolic acid B was determined by HPLC. To identify Panax notoginseng, samples were first washed with water and 30% methanol to reduce the interference of impurities and then eluted with methanol. The eluate was eluted with dichloromethane-absolute ethanol-water (70: 45: 6.5) TLC as a developing agent, the results can notoginseng saponins R1, ginsenosides Re, Rb1 and Rg1 effective separation. Determination of salvianolic acid B content, the use of C18 column with methanol - acetonitrile - formic acid - water (30: 10: 1: 59) as the mobile phase, detection wavelength of 286 nm. Salvianolic acid B has good linearity in the range of 0.1 ~ 10 g. The qualitative and quantitative methods established in this study are simple, accurate and specific, which can effectively control the quality of compound Danshen capsules.