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目的探讨康复训练对大鼠脑出血后室管膜下区(SVZ)神经干细胞(NSCs)增殖和分化的影响。方法 75只SD大鼠随机分为康复组、制动组和假手术组(每组25只),康复组和制动组用胶原酶诱导脑出血模型,假手术组用生理盐水替代胶原酶。康复组每天予以抓握、平衡、旋转等训练,制动组置于网状笼内固定,假手术组在笼内自由活动。每组大鼠康复前后进行神经功能评分,分别于康复训练后1、4、7、14和28天处死动物。应用免疫组织化学方法检测大鼠SVZ神经细胞巢蛋白(Nestin)和5-溴脱氧尿核苷(BrdU)表达的变化。结果康复组神经功能缺损评分低于制动组(P<0.05)。康复组大鼠SVZ BrdU阳性、Nestin阳性细胞在不同时间点均多于制动组大鼠(P<0.05),第7天康复组SVZ BrdU阳性、Nestin阳性细胞表达达到高峰,14天时表达逐渐减少(P<0.05,P<0.01)。结论康复训练可促进脑出血后大鼠NSCs增殖,并向神经元分化。
Objective To investigate the effects of rehabilitation training on the proliferation and differentiation of neural stem cells (NSCs) in the subventricular zone (SVZ) after intracerebral hemorrhage in rats. Methods Seventy-five SD rats were randomly divided into rehabilitation group, braking group and sham operation group (25 rats in each group). Rats in rehabilitation group and braking group were induced by collagenase. Rats in sham operation group were given saline instead of collagenase. Rehabilitation group was grasped every day, balance, rotation and other training, brake group placed in a mesh cage fixed, sham group in the cage free movement. Neurological scores were assessed before and after rehabilitation in each group of rats, and animals were sacrificed at 1, 4, 7, 14 and 28 days after rehabilitation. Immunohistochemistry was used to detect the expression of nestin and BrdU in SVZ neurons. Results The scores of neurological deficits in rehabilitation group were lower than those in brachial group (P <0.05). SVZ BrdU positive and Nestin positive cells in rehabilitation group were higher than those in brachial group at different time points (P <0.05). On the 7th day, SVZ BrdU positive and Nestin positive cells reached the peak in the rehabilitation group, and gradually decreased on the 14th day (P <0.05, P <0.01). Conclusion Rehabilitation can promote the proliferation of rat NSCs and differentiate into neurons after intracerebral hemorrhage.