论文部分内容阅读
目的:对吉林省9个地区的媒介蜱、野鼠、家犬进行伯氏疏病原体分离与检测。方法:应用BSK培养基、直接免疫荧光抗体染色法(DFA)进行蜱、动物螺旋体培养、检测。结果:媒介蜱株螺旋体带菌率为35.80%(1278/3570)。黑线姬鼠带菌率为21.91(64/292)、褐家属18.90(31/164)、黑线仓鼠13.44(34/253),3种鼠共检测了709只,平均带菌率为15.92(107/709)。其它棕背鼠、小家鼠等110只,平均带菌率为12.73(14/110)。家犬检出率为8.46%(23/272)。结论:检验结果显示:吉林省9个地区媒介蜱带菌率均有显著性差异。动物感染率:牛山区与半山区、家犬山区与平原P值<0.05均有显著性差异。其他均无显著性差异。
OBJECTIVE: To isolate and detect Beauveria bassiana pathogens from ticks, wild rats and domestic dogs in 9 regions of Jilin Province. Methods: Bovine ticks and animals were cultured and detected by using BSK medium and direct immunofluorescence staining (DFA). Results: The transmission rate of spirochetes of vector ticks was 35.80% (1278/3570). The incidences of fungi in Apodemus agrarius were 21.91 (64/292), 18.90 (31/164) in Brown family and 13.44 (34/253) in Black hamster, 709 were detected in 3 species of mice, with an average carrier rate of 15.92 (107 / 709). Other brown mice, Mus musculus and other 110, the average carrying rate of 12.73 (14/110). The detection rate of domestic dogs was 8.46% (23/272). Conclusion: The test results show that there are significant differences in the carrying rate of vector ticks in nine areas in Jilin Province. Animal infection rate: Niu Mountain and Mid-levels, domestic dogs mountain and plain P value <0.05 were significantly different. Other no significant difference.