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目的:研究快速老化P8小鼠(senescence accelerated mouse-prone/8,SAM-P8)痕迹性眨眼条件反射(trace eyeblink conditioning,TEBC)的获得及其前额叶(prefrontal cortex,PFC)事件相关电位(event related potential,ERP)的变化。方法:实验动物为SAM-P8组(n=11)与对照小鼠SAM-R1(senescence accelerated mouse-resistant/1,SAM-R1)组(n=11);对2组小鼠进行条件刺激(conditioned stimulus,CS)与非条件刺激(unconditioned stimulus,US)配对的经典TEBC训练,其中CS为250 ms、3 k Hz、85 d B的纯音,US为强度为3 Psi、持续时间100 ms的束状氧气对动物角膜进行给气。观察2组动物的经典TEBC的条件反应(conditioned response,CR)的习得及其PFC-ERP的变化。结果:行为学结果方面,SAM-P8可以获得有限的经典TEBC(CS:250ms 85 d B的纯音),SAM-R1的条件反应发生率(incidence rate of conditioned response,CR%)明显高于SAM-P8(P<0.05)。PFCERP结果方面:在CR期,无论是峰峰幅度和峰潜伏期未见SAM-P8与SAM-R1 2组间有明显差别,且2组组内训练各期之间没有统计学差异(P>0.05);在CR前期,SAM-P8的PFC-ERP的峰峰幅度显著低于SAM-R1(P<0.01),SAM-R1行为习得中期PFC-ERP的峰峰幅度明显高于其行为习得早期(P<0.05),但SAM-P8未见此显著变化。结论:SAM-P8小鼠对经典TEBC的学习能力有损伤但并不完全;在TEBC建立过程中,SAM-P8小鼠其前额叶的参与程度低于SAM-R1小鼠,PFC可能更多参与TEBC的中期获得过程,但这一时间特征对于SAM-P8似乎并不明显。
OBJECTIVE: To study the acquisition of trace eyeblink conditioning (TEBC) in senescence accelerated mouse-prone / 8 (SAM-P8) and its related potential of prefrontal cortex (PFC) event related potential, ERP) changes. Methods: The experimental animals were treated with SAM-P8 (n = 11) and control mice with SAM-R1 (n = 11) conditioned stimulus (CS) training with classical unconjugation stimuli (US), where CS is pure tone of 250 ms, 3 k Hz, 85 d B and US is pure intensity of 3 Psi with a duration of 100 ms Oxygen gives gas to animal cornea. The conditioned response (CR) acquisition and PFC-ERP changes of classical TEBC in two groups of animals were observed. RESULTS: In terms of behavioral results, SAM-P8 was able to obtain limited classical TEBC (CS: pure tone of 85 ms 85 d B), and the incidence rate of conditioned response (CR%) of SAM-R1 was significantly higher than that of SAM- P8 (P <0.05). In the PFCERP results, there was no significant difference between the two groups during the CR phase (P> 0.05 for both peak amplitude and peak latency) for both SAM-P8 and SAM-R1 groups (P <0.01). The peak-to-peak amplitude of PFC-ERP in SAM-P8 was significantly higher than that in SAM-R1 (P <0.01) Early (P <0.05), but no significant change in SAM-P8. Conclusions: The learning ability of classic TEBC is damaged but not complete in SAM-P8 mice. The involvement of prefrontal cortex in SAM-P8 mice is lower than that in SAM-R1 mice during TEBC establishment, and PFC may be more involved TEBC medium-term acquisition, but this time characteristic does not seem obvious for SAM-P8.