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目的探索溶血标本对化学发光方法检测献血者四项传染病指标的影响。方法无偿献血者乙型肝炎病毒表面抗原(HBs Ag)、丙型肝炎病毒抗体(抗-HCV)、梅毒螺旋体抗体(抗-TP)单项检测结果阳性标本各30份,人类免疫缺陷病毒抗体(抗-HIV)检验结果阳性标本10份及随机选取四项传染病检测结果均阴性的标本30份。将实验分为4组,分为原血清组、轻度、中度和重度溶血组。原血清组标本不做任何处理,人为造成轻、中、重度溶血,血红蛋白浓度≤2 g/L为轻度溶血组,2 g/L<血红蛋白浓度≤4 g/L为中度溶血组,血红蛋白浓度>4 g/L为重度溶血组。在全自动免疫分析仪上进行了化学发光法检测。结果轻度、中度、重度溶血组HBs Ag、抗-HCV、抗-HIV和抗-TP的测定值与原血清组比较差异均无统计学意义(P>0.05)。结论溶血标本用化学发光法检测HBs Ag、抗-HCV、抗-HIV和抗-TP的结果没有明显影响,化学发光法可用于献血者传染病四项筛查。
Objective To explore the influence of hemolytic samples on the detection of four infectious diseases by chemiluminescence method. Methods Thirty healthy donors were immunized with HBs Ag, anti-HCV and anti-TP, and 30 were positive for anti-HCV, anti-HIV and anti- -HIV) 10 positive specimens and 30 randomly selected specimens with negative results of four infectious diseases. The experiment was divided into 4 groups, divided into the original serum group, mild, moderate and severe hemolysis group. The original serum group did not do any treatment, artificially caused by light, moderate and severe hemolysis, hemoglobin concentration ≤ 2 g / L mild hemolytic group, 2 g / L hemoglobin concentration ≤ 4 g / L moderate hemolytic group, hemoglobin Concentration> 4 g / L severe hemolysis group. Chemiluminescence detection was performed on a fully automatic immunoassay analyzer. Results The levels of HBsAg, anti-HCV, anti-HIV and anti-TP in mild, moderate and severe hemolysis group were not significantly different from the original serum group (P> 0.05). Conclusion The results of chemiluminescence assay for detection of HBsAg, anti-HCV, anti-HIV and anti-TP in hemolytic samples have no significant effect, and chemiluminescence method can be used in four screening of infectious diseases of blood donors.