论文部分内容阅读
目的:探讨“高住低练”肝细胞凋亡调控基因,凋亡调控基因与HIF-1α之间关系。方法:健康成年雄性SD大鼠60只,随机分为6组。低氧暴露组(HE)和高住低练组(Hilo)每天低氧暴露(氧浓度12.6%,相当于海拔4000m)8h和12h,5d/w,共4w;常氧运动组和高住低练组每天均以25m/min的速度训练1h,5d/w,共4w。采用免疫组织化学方法和计算机显微图像分析系统分析bax、bcl-2和HIF-1α阳性表达、阳性物质的定位及定量。结果:(1)Hilo组的bax蛋白表达与HE组和T组相比显著增加(P<0.05),随着低氧暴露时间的延长,呈增长趋势;TUNEL与bax呈正相关(r=0.693,P<0.01);(2)HE组bcl-2蛋白表达显著高于C组,12hHE组的bcl-2蛋白表达比8hHE组显著下降(P<0.05);12Hilo组bcl-2蛋白表达比8Hilo组显著下降,但明显高于C组(P<0.05);(3)肝组织bax/bcl-2值显示,C组与12hHE组和12Hilo组相比具有显著性意义P<0.05);T组与Hilo组相比具有非常显著性意义(P<0.01);(4)HIF-1α与bax呈高度正相关(r=0.958,P<0.01)。结论:bax、bcl-2与HIF-1α基因参与调控肝细胞的凋亡;HIF-1α可能调控bax和bcl-2,并与bax呈高度正相关,调控bax高表达而促进肝细胞凋亡。
OBJECTIVE: To investigate the relationship between apoptosis-controlling genes and apoptosis-controlling genes of hepatocyte apoptosis in “Living High and Training Low”. Methods: Sixty healthy adult male Sprague-Dawley rats were randomly divided into 6 groups. The hypoxia exposure (12.6% oxygen, equivalent to an altitude of 4000m) was observed for 8h, 12h and 5d / w in the hypoxia exposure group (Hilo) and hyperoxia group (Hilo) Training group every day at 25m / min speed training 1h, 5d / w, a total of 4w. The expression of bax, bcl-2 and HIF-1α, the localization and quantification of positive substances were analyzed by immunohistochemistry and computerized microscopic image analysis system. Results: (1) The expression of bax protein in Hilo group was significantly higher than that in HE group and T group (P <0.05), and increased with the prolongation of hypoxia exposure. TUNEL was positively correlated with bax (r = 0.693, P <0.01). (2) The expression of bcl-2 protein in HE group was significantly higher than that in C group. The expression of bcl-2 protein in 12hHE group was significantly lower than that in 8hHE group (P <0.05). (3) The expression of bax / bcl-2 in liver tissue in group C was significantly higher than that in group 12HHE and group 12Hilo (P <0.05) Hilo group (P <0.01). (4) There was a positive correlation between HIF-1α and bax (r = 0.958, P <0.01). CONCLUSION: Bax, bcl-2 and HIF-1α genes are involved in the regulation of apoptosis in hepatocytes. HIF-1α may regulate bax and bcl-2, which are highly positively correlated with bax and regulate bax high expression to promote hepatocyte apoptosis.