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近年来,由于世界人口的老龄化及许多疾病的不可治愈性,成人间充质干细胞(hMSCs)用于细胞治疗越来越受到重视。虽然成人间充质干细胞可来源于骨髓、脂肪、脐血、脐带、胎盘、骨骼肌等,但由于其细胞数目较少,在临床运用之前必须依靠体外扩增来获取理想的细胞数量。本文拟搜索前期研究来探讨人间充质干细胞体外扩增期间影响细胞数目的因素,如供体的年龄、性别、组织来源选择及细胞接种密度,从而优化获取足量人间充质干细胞的过程,进而为今后临床干细胞治疗提供必要的理论基础。
In recent years, due to the aging of the world’s population and the incurability of many diseases, adult mesenchymal stem cells (hMSCs) are gaining more and more attention for cell therapy. Although adult mesenchymal stem cells can be derived from bone marrow, adipose tissue, umbilical cord blood, umbilical cord, placenta, skeletal muscle and so on, due to their small number of cells, they must rely on in vitro amplification to obtain the ideal cell number before clinical application. This study was to search for early-stage studies to explore the factors that influence the number of cells during in vitro expansion of human mesenchymal stem cells, such as the donor’s age, sex, tissue source selection, and cell seeding density to optimize access to adequate quantities of mesenchymal stem cells For future clinical stem cell therapy to provide the necessary theoretical basis.