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目的观察心钠素对豚鼠耳蜗缺血再灌注损伤的影响。方法将豚鼠分为4组:实验组(A1、B1)及对照组(A2、B2)。采用造血栓后溶栓的方法制备耳蜗缺血再灌注模型。实验组A1在建模前10 m in静脉注射心钠素,实验组B1在再灌注即刻静脉注射心钠素,对照组(A2、B2)在相应时间静脉注射等量生理盐水。实验过程中采用激光多普勒血流量仪监测耳蜗血流量(cochlear b loodflow,CoBF)并测定豚鼠听性脑干反应(aud itory brainstem response,ABR)值。结果缺血前实验组A1的CoBF较对照组A2高,再灌注后至实验结束,2组CoBF值未见明显差别。实验组B1和对照组B2用药前的CoBF无明显差别,再灌注后对照组B2恢复到实验开始时的70%左右,而实验组B1恢复到实验开始时相同水平。缺血前4组听阈差异无统计学意义。缺血30 m in时,实验组A1的听阈较对照组A2低(t=7.761,P<0.05)。实验组B1和对照组B2听阈差异无统计学意义。再灌注30 m in和60 m in时,实验组A1与对照组A2差异无统计学意义。实验组B1比对照组B2显著降低(t值分别为9.846和19.242,P值均<0.05)。结论缺血再灌注后即刻应用心钠素,可以减轻耳蜗的缺血再灌注损伤,可以在增加耳蜗血流的同时降低听反应阈。为临床内耳微循环障碍疾病提供一种新的药物治疗方法。
Objective To observe the effect of atrial natriuretic peptide on the cochlear ischemia-reperfusion injury in guinea pigs. Methods Guinea pigs were divided into 4 groups: experimental group (A1, B1) and control group (A2, B2). The cochlear ischemia-reperfusion model was prepared by thrombolysis after thrombosis. In experimental group A1, atrial natriuretic peptide was injected intravenously 10 min before modeling, and in experimental group B1, atrial natriuretic peptide was injected intravenously immediately after reperfusion. In the control group (A2 and B2), normal saline was injected intravenously at the corresponding time points. During the experiment, cochlear blood flow (CoBF) was monitored by laser Doppler flowmetry, and the auditory brainstem response (ABR) was measured in guinea pigs. Results Before ischemia, the CoBF of experimental group A1 was higher than that of control group A2, and no significant difference was found between the two groups after reperfusion. There was no significant difference in CoBF between experimental group B1 and control group B2 before treatment. After reperfusion, B2 in control group returned to about 70% at the beginning of experiment, while experimental group B1 returned to the same level at the beginning of experiment. There was no significant difference in hearing threshold between the 4 groups before ischemia. At 30 min of ischemia, the hearing threshold of experimental group A1 was lower than that of control group A2 (t = 7.761, P <0.05). There was no significant difference in hearing threshold between experimental group B1 and control group B2. There was no significant difference between experimental group A1 and control group A2 when reperfused for 30 mins and 60 mins. The experimental group B1 was significantly lower than the control group B2 (t = 9.846 and 19.242, P <0.05 respectively). Conclusion Atrial Natriuretic Peptide can reduce ischemia-reperfusion injury immediately after ischemia-reperfusion, reduce the threshold of hearing reaction while increasing cochlear blood flow. For the clinical inner ear microcirculation disorders provide a new drug treatment.