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目的:为提高食品中转基因成分检测的灵敏度,将微流控芯片仪应用到转基因检测中。方法:将微流控芯片法与普通PCR法、荧光定量PCR法进行灵敏度比较;检测高温、高压处理过的转基因大豆粉。结果:荧光定量PCR法的灵敏度明显高于普通PCR法,但在较极端高温、高压处理(高压灭菌121℃/30 min)及转基因成分含量较低(1%)的情况下,采用荧光定量PCR法未能检测到外源基因,而微流控芯片法能检测到相应的峰值,前提是在未添加内标(Marker)的情况下。若添加内标,在内标峰和目的峰区分开的条件下,有损检测灵敏度。结论:微流控芯片在转基因检测中具有高效率、高灵敏度的特点,但该方法有待完善。
OBJECTIVE: To improve the sensitivity of detection of GMO in food, microfluidic chip was applied to the detection of GMO. Methods: The sensitivity of microfluidic chip method was compared with that of ordinary PCR method and fluorescence quantitative PCR method. The high temperature and high pressure treated soybean powder was tested. Results: The sensitivity of real-time fluorescence quantitative PCR was significantly higher than that of ordinary PCR, but under the conditions of extreme high temperature and high pressure (121 ℃ / 30 min of autoclaving) and low (1%) content of GMO, PCR method failed to detect exogenous gene, and the microfluidic chip method can detect the corresponding peak, provided that no addition of an internal standard (Marker) case. If an internal standard is added, the sensitivity of detection will be deteriorated under the condition that the internal standard peak and the target peak area are separated. Conclusion: Microfluidic chips have the characteristics of high efficiency and high sensitivity in the detection of transgenic products, but the method needs to be improved.