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利用叠氮溴化丙锭与聚合酶链式反应结合(PMA-PCR),建立了西瓜细菌性果斑病病菌(Acidovorax avenae subsp.citrulli)活细胞检测方法。实验结果表明,当PMA浓度为0.5 mg/m L时,对活细胞DNA的PCR扩增无影响,最低检测浓度为1×104cfu/m L。当样品中死亡细胞浓度低于107 cfu/m L时,PMA可抑制死亡细胞DNA的PCR扩增;当死亡细胞浓度为1×107cfu/m L时,PMA失去效果。该方法适用于低浓度西瓜细菌性果斑病病菌活细胞的检测。
The method for detecting viable cells of Acidovorax avenae subsp. Citrulli was established by the combination of azidopropyl bromide and polymerase chain reaction (PMA-PCR). The experimental results showed that when the concentration of PMA was 0.5 mg / m L, there was no effect on the PCR amplification of live DNA, the lowest detection concentration was 1 × 10 4 cfu / m L. When the concentration of dead cells in the sample was less than 107 cfu / m L, PMA inhibited the DNA amplification of dead cells. When the concentration of dead cells was 1 × 107 cfu / m L, PMA lost its effect. The method is suitable for the detection of living cells of low-concentration watermelon bacterial fruit-leaf spot bacteria.