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目的探讨视网膜母细胞瘤结合蛋白4(retinoblastoma binding protein 4,RBBP4)和BCL-2在胃癌中的表达及其与临床病理特征的关系。方法采用免疫组化SP法检测72例胃癌及对应癌旁组织中RBBP4、BCL-2蛋白表达,分析RBBP4与胃癌临床病理特征的关系及与BCL-2表达的相关性。实时荧光定量PCR法检测20例胃癌及癌旁组织中RBBP4 mRNA的表达。结果 RBBP4、BCL-2蛋白在癌组织中的阳性率分别为55.6%(40/72)、50%(36/72),显著高于癌旁组织13.9%、16.7%。RBBP4和BCL-2高表达均与肿瘤分化、浸润深度及淋巴结转移明显相关(P<0.05),且RBBP4和BCL-2蛋白的表达呈正相关(r=0.559,P<0.05)。RBBP4 mRNA在胃癌组织中的相对表达水平(9.988±1.948)显著高于癌旁组织(1.458±0.489)。结论RBBP4在胃癌中的表达升高,可能和胃癌的发生、发展及侵袭转移有关,可能成为胃癌诊断和预后评估的新指标。
Objective To investigate the expression of retinoblastoma binding protein 4 (RBBP4) and BCL-2 in gastric cancer and its relationship with clinicopathological features. Methods The expressions of RBBP4 and BCL-2 protein in 72 cases of gastric cancer and its corresponding paracancerous tissues were detected by immunohistochemical SP method. The relationship between RBBP4 and the clinicopathological characteristics of gastric carcinoma and the expression of BCL-2 were analyzed. Real-time quantitative PCR was used to detect the expression of RBBP4 mRNA in 20 cases of gastric cancer and its adjacent tissues. Results The positive rates of RBBP4 and BCL-2 protein in cancer tissues were 55.6% (40/72) and 50% (36/72), respectively, which were significantly higher than those in paracancerous tissues (13.9% and 16.7%). The high expressions of RBBP4 and BCL-2 were significantly correlated with tumor differentiation, depth of invasion and lymph node metastasis (P <0.05), and the expressions of RBBP4 and BCL-2 were positively correlated (r = 0.559, P <0.05). The relative expression level of RBBP4 mRNA in gastric cancer tissues (9.988 ± 1.948) was significantly higher than that in paracancerous tissues (1.458 ± 0.489). Conclusion The expression of RBBP4 in gastric cancer may be related to the occurrence, development and invasion and metastasis of gastric cancer, which may be a new indicator for the diagnosis and prognosis evaluation of gastric cancer.