论文部分内容阅读
目的探索不同的维甲酸(retinoic acid,RA)剂量对小鼠神经系统发育的影响以及在特异时间点作用于小鼠胚胎时对神经管闭合的影响。方法采用C57孕鼠于GD7.5分别腹部注射5mg/kg,7.5mg/kg,20mg/kg RA,于GD10.5腹部注射20mg/kg RA,观察胎鼠表型并称量胎鼠重量,胎脑重量,测量眼距和顶距(前囟到鼠喙的距离)。结果 GD7.5腹部注射5mg/kg RA的胎鼠出现无眼畸形,7.5mg/kg的胎鼠出现脑膨出,20mg/kg的吸收胎率为100%,GD10.5腹部注射20mg/kg RA的胎鼠前肢短小其中,GD7.5给予7.5mg/kg RA处理后的胎鼠脑重显著低于正常组和其他实验组;GD7.5时给予7.5mg/kg RA处理组及GD10.5时20mg/kg RA处理组的胎鼠重量显著低于正常组;GD7.5时5mg/kg RA处理组的胎鼠顶距低于正常组。结论在胚胎发育的不同时期暴露于过量的RA会影响不同器官的发育,而且RA对颅面畸形的影响呈剂量依赖性。
Objective To explore the effects of different doses of retinoic acid (RA) on the development of nervous system in mice and the effect on the closure of neural tube when acting on mouse embryos at specific time points. Methods C57 pregnant mice were injected intraperitoneally with 5mg / kg, 7.5mg / kg, 20mg / kg RA respectively on GD7.5, and injected with 20mg / kg RA on the abdomen of GD10.5 to observe the fetal rat phenotype and weigh the fetal rat Brain weight, distance and distance measured (distance between bregma and rat beaks). Results There was no ocular deformity in fetus with GD7.5 5 mg / kg RA. The fetus with 7.5 mg / kg had bulging encephalocele, 100% with 20 mg / kg and GD 20 with 20 mg / kg RA Of fetal rat short forelimbs, GD7.5 given 7.5mg / kg RA treatment of fetal rat brain weight was significantly lower than the normal group and other experimental groups; GD7.5 7.5mg / kg RA treatment group and GD10.5 The weight of fetal rats in the 20 mg / kg RA group was significantly lower than that in the normal group, while that of the 5 mg / kg RA group in the GD7.5 group was lower than that of the normal group. Conclusion Exposed to excessive RA during different stages of embryo development may affect the development of different organs, and the effect of RA on craniofacial deformity is dose-dependent.