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番茄黄化曲叶病毒病(tomato yellow leaf curl virus disease,TYLCD)是番茄生产上最严重的病害之一。为了更有效地分析其致病机理,本研究根据已知的番茄黄化曲叶病毒(tomato yellow leaf curl virus,TYLCV)基因组序列设计引物,通过PCR扩增技术,分离浙江省TYLCV的全长序列,分析其结构特征,序列同源性及系统发育关系。结果表明:病毒基因全长为2781个核苷酸,共编码6个开放阅读框(AV1,AV2,AC3,AC2,AC1,AC4),即TYLCV的典型结构;核苷酸序列比对分析发现该病毒分离物与美国的TYLCV同源性最高(99.6%);6个编码区分析显示除云南外,AV2与国内其他地区同源性均高达100%;系统发育关系分析表明病毒分离物与江苏、北京、美国和墨西哥的TYLCV划分为一类,亲缘关系最近。这些结果为今后揭示TYLCV的致病机理奠定基础,并为番茄黄化曲叶病毒病的诊断和防治提供科学依据。
Tomato yellow leaf curl virus disease (TYLCD) is one of the most serious diseases in tomato production. In order to analyze the pathogenesis of TYLCV more efficiently, we designed primers based on the known TYLCV genome sequence of TYLCV, and isolated the full-length TYLCV sequence of Zhejiang province by PCR amplification , Analyzed its structural features, sequence homology and phylogenetic relationships. The results showed that the full length of the virus gene was 2781 nucleotides, encoding a total of six open reading frames (AV1, AV2, AC3, AC2, AC1, AC4), TYLCV typical structure; nucleotide sequence alignment analysis found that The virus isolates had the highest homology with TYLCV in the United States (99.6%). The analysis of 6 coding regions showed that except for Yunnan, AV2 shared 100% homology with other regions in China. The phylogenetic relationship analysis indicated that the virus isolates were highly homologous with Jiangsu, The TYLCVs in Beijing, the United States and Mexico are grouped into one category, most recently related. These results lay the foundation for revealing the pathogenesis of TYLCV in the future and provide a scientific basis for the diagnosis and prevention of tomato yellow leaf curl virus disease.