论文部分内容阅读
为了研究感染副溶血弧菌对斜带石斑鱼体内一氧化氮合酶活力的影响 ,分别对斜带石斑鱼腹腔注射浓度为 5× 10 8cfu/ml、5× 10 7cfu/ml、5× 10 6cfu/ml的副溶血弧菌悬浮液 ,在注射后 2 4h、4 8h、72h、12 0h和 192h尾部取血 ,测定其血清中NOS活力的变化情况。结果表明 ,注射副溶血弧菌后斜带石斑鱼血清中NOS活力显著高于对照组 ,并且高浓度组NOS活力显著高于低浓度组 ,证明注射副溶血弧菌可以诱导斜带石斑鱼体内NOS活力的升高。
In order to study the effect of Vibrio parahaemolyticus on the nitric oxide synthase activity of the grouper, the effects of intraperitoneal injection of 5 × 10 8 cfu / ml, 5 × 10 7 cfu / ml, 10 6 cfu / ml suspension of V. parahaemolyticus, blood was taken from the tail of the mice at 24 h, 48 h, 72 h, 120 h and 192 h after injection, and the changes of NOS activity in serum were measured. The results showed that the activity of NOS in Serratia glabra after injection of Vibrio parahaemolyticus was significantly higher than that of the control group, and the activity of NOS in high concentration group was significantly higher than that in the low concentration group. It was proved that Vibrio parahaemolyticus could induce the growth of oblique grouper NOS activity in vivo increased.