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目的：探讨多发性骨髓瘤（ＭＭ）患者骨髓细胞中白细胞介素６（ＩＬ－６）分泌机制。方法：应用双标记免疫荧光技术检测了１６例ＭＭ患者和１９例对照者新鲜分离的骨髓单个核细胞中ＩＬ－６表达情况。结果：ＭＭ患者骨髓单个核细胞ＩＬ－６表达阳性率为１５．４％±６．５％，骨髓瘤细胞ＩＬ－６表达阳性率达７．９％±２．９％，基质细胞ＩＬ－６表达阳性率达４．５％±１．２％；对照组骨髓单个核细胞ＩＬ－６表达阳性率为４．６％±２．３％，基质细胞ＩＬ－６表达阳性率为２．６％±１．１％，浆细胞ＩＬ－６表达阳性率为０．７％±０．１％。ＭＭ患者均较对照组显著升高（Ｐ均＜０．００１）。另外ＭＭ组骨髓单个核细胞中基质细胞所占比率为６．９％±１．４％，也较对照组的４．７％±１．８％明显升高（Ｐ＜０．００１）。结论：ＭＭ患者升高的ＩＬ－６既可由瘤细胞分泌，又可由基质细胞分泌，自分泌和旁分泌机制可能都参与了ＭＭ的发病。
Objective: To investigate the mechanism of interleukin 6 (IL-6) secretion in bone marrow cells of patients with multiple myeloma (MM). METHODS: IL-6 expression in bone marrow mononuclear cells freshly isolated from 16 MM patients and 19 controls was detected by dual-labeled immunofluorescence. Results: The positive rate of IL-6 expression in bone marrow mononuclear cells in MM patients was 15.4%±6.5%. The positive rate of IL-6 expression in myeloma cells was 7.9%±2.9%. The stromal cells IL-6 The positive rate of expression was 4.5%±1.2%. The positive rate of IL-6 expression in bone marrow mononuclear cells in control group was 4.6%±2.3%. The positive rate of IL-6 expression in stromal cells was 2.6%. ±1.1%, positive rate of plasma cell IL-6 expression was 0.7%±0.1%. MM patients were significantly higher than the control group (P all < 0.001). In addition, the ratio of stromal cells in bone marrow mononuclear cells in the MM group was 6.9%±1.4%, which was also significantly higher than that in the control group (4.7%±1.8%) (P<0.001). Conclusion: The elevated IL-6 in MM patients can be secreted by tumor cells and secreted by stromal cells. Both autocrine and paracrine mechanisms may participate in the pathogenesis of MM.