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目的 :分析染色体 9p2 1.3- 2 3区域等位基因杂合性缺失 (lossofheterozygosity ,LOH)情况 ,确定其在中国人喉鳞癌中发生频率和最小缺失区域 ,为定位克隆喉鳞癌相关抑癌基因提供线索。方法 :应用 9个微卫星多态性标记对 2 4例喉鳞癌组织及其对应的正常远端肺组织进行了同位素标记的聚合酶链反应和变性凝胶电泳 ,并探讨各位点LOH与病理分级和病理分期的关系。结果 :2 4例喉鳞癌组织中 ,共有 2 3例 (95 .83% )存在至少一个位点的LOH ,丢失频率最高的位点位于9p2 2 - 2 3的D9S1782 (10 0 % )其次是位于 9p2 2 - 2 3的D9S16 2 (85 .71% )。结论 :在 9p2 1.3- 2 3区域可能存在两个或两个以上的与喉鳞癌发生相关的候选抑癌基因 ,分别位于 9p2 2 - 2 3的D9S1782和D9S16 2附近
OBJECTIVE: To analyze the loss of heterozygosity (LOH) in the 9p2 1.3-2 3 region of chromosome to determine its frequency and minimal deletion region in Chinese laryngeal squamous cell carcinoma. In order to locate the tumor suppressor gene associated with laryngeal squamous cell carcinoma Provide clues. Methods: Nine microsatellite polymorphism markers were used to perform polymerase chain reaction and denaturing gel electrophoresis on 24 cases of laryngeal squamous cell carcinoma and their corresponding normal distal lung tissues. The relationship between LOH and pathology Grading and pathological staging of the relationship. RESULTS: Twenty - three cases (95.83%) had LOH in at least one locus in 24 cases of laryngeal squamous cell carcinoma, and the sites with the highest frequency of loss were D9S1782 (10 0%) in 9p2 2 - 2 3 D9S16 2 at 9p2 2 - 2 3 (85.71%). CONCLUSION: There may be two or more candidate tumor suppressor genes associated with the occurrence of laryngeal squamous cell carcinoma in 9p2 1.3 - 2 3 region, located in the vicinity of D9S1782 and D9S16 2 of 9p2 2 - 2 3