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本文旨在研究无远处转移的结直肠癌病人NK细胞活性在根治性术前后的动态变化。 40例病理证实的结直肠癌病人,按美国癌症联合委员会/国际抗癌联盟标准Ⅰ期12例,Ⅱ期15例,Ⅲ期13例行根治切除术。分别于术前2天、术后即时、术后1天、7天、21天各取静脉血20ml分离NK细胞,培养并稀释至10~7细胞/ml作为效应细胞。以~(51)Cr标记的K562细胞为靶细胞。二者共同培养5小时,靶细胞裂
This article aims to investigate the dynamic changes of NK cell activity in patients with colorectal cancer without distant metastasis before and after radical surgery. Forty patients with pathologically confirmed colorectal cancer underwent radical resection in accordance with the United States Joint Committee on Cancer/International Anticancer Union Standard I, 12 cases, Stage II, 15 cases, and Stage III, 13 cases. NK cells were isolated from 20 ml of venous blood 2 days before surgery, immediately after surgery, 1 day, 7 days, and 21 days after surgery, and cultured and diluted to 10-7 cells/ml as effector cells. K562 cells labeled with ~(51)Cr were used as target cells. The two are co-cultured for 5 hours and the target cells are cleft