目的:研究HCA-Ⅱ肾保存液对草犬肾脏低温保存的效果。方法:采用草犬肾脏非循环离体灌注模型,比较HCA-Ⅱ液、UW液和高渗枸橼酸盐嘌呤溶液(HC-A液)低温保存肾脏后其形态学及生物化学指标的改变,并进行自体肾脏移植,观察移植后肾功能恢复情况。结果:HCA-Ⅱ液组保存48、72 h后,形态学改变、肾脏线粒体呼吸控制率(RCR)和皮质ATP含量、移植肾功能指标明显优于HC-A液(P<0.05),与UW液组无明显差异;低温保存供肾24 h,3组上述指标无显著差异。保存24、48、72 h后保存液pH值,HCA-Ⅱ液组均明显高于HC液组(P<0.05)。结论:HCA-Ⅱ液保存犬肾48 h及72 h效果优于HC-A液,与UW液相当。 OBJECTIVE: To study the effect of HCA-Ⅱ renal preservation solution on the preservation of the canine kidney at low temperature. Methods: The non-circulatory instillation model of canine kidney was used to compare the morphological and biochemical indexes of HCA-Ⅱ fluid, UW fluid and hypertonic citrate purine solution (HC-A fluid) And autologous kidney transplantation to observe the recovery of renal function after transplantation. Results: The morphological changes, mitochondrial respiratory control rate (RCR) and cortical ATP content of HCA-Ⅱ fluid group were significantly better than that of HC-A fluid group (P <0.05) at 48 and 72 h, Liquid group no significant difference; low temperature preservation of the kidneys for 24 h, three groups of the above indicators no significant difference. After 24, 48 and 72 h storage, the pH value of the preservation solution was significantly higher in the HCA-Ⅱ fluid group than in the HC fluid group (P <0.05). CONCLUSION: HCA-Ⅱ fluid is superior to HC-A solution in preserving canine kidney at 48 h and 72 h, comparable to UW solution.