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目的:探讨乌司他丁对脓毒症大鼠神经功能的保护作用方法:46只SD大鼠随机分为空白对照组(Norm组,n=8)、假手术组(Sham组,n=8)和盲肠结扎穿孔组(CLP组,n=30),仅CLP组进行盲肠结扎穿孔术(cecal ligation and puncture,CLP)。24 h后评估各组的神经功能缺损评分(neurological deficient score,NDS),将脓毒症动物(S~+)随机分为S~+-control组和S~+-UTI组,检测Norm组、Sham组、S~+-control组和S~+-UTI组血清S100β的含量,随后腹腔注射乌司他丁(Ulinastatin,UTI)10万U/kg(tid,6 h/次)或等体积生理盐水。干预后24 h再次评估NDS,检测血清S100β的含量。结果:CLP术后24 h,约35%(10/29)的大鼠未发展为脓毒症;与Sham组相比,S~+的NDS明显降低(P<0.01),血清S100β明显升高(P<0.01)。干预后24 h,S~+-UTI组的NDS高于S~+-control组(P=0.019),而血清S100β低于S~+-control组(P=0.015)。结论:乌司他丁对脓毒症大鼠的神经功能具有保护作用。
Objective: To investigate the protective effects of ulinastatin on neurological function in septic rats.Methods: Forty-six SD rats were randomly divided into blank control group (Norm group, n = 8), sham operation group (n = 8 ) And cecal ligation and puncture group (CLP group, n = 30). CLP group only received cecal ligation and puncture (CLP). The neurological deficient score (NDS) of each group was evaluated 24 h later. The sepsis animals (S ~ +) were randomly divided into S ~ + -control group and S ~ + -UTI group. The Norm group, Sham group, S ~ + -control group and S ~ + -UTI group, and then intraperitoneal injection of Ulinastatin (UTI) 100000 U / kg (tid, 6 h / times) or the same volume of physiology brine. 24 h after intervention, NDS was re-evaluated, and serum S100β level was measured. Results: About 35% (10/29) of rats did not develop sepsis at 24 h after CLP. Compared with Sham group, the NDS of S ~ + was significantly lower (P <0.01) and S100β was significantly higher (P <0.01). At 24 h after intervention, NDS in S ~ + -UTI group was higher than that in S ~ + -control group (P = 0.019), while serum S100β was lower than that in S ~ + -control group (P = 0.015). Conclusion: Ulinastatin can protect the neurological function of septic rats.