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海马内移植内嗅皮层的大鼠脑经高浓度戊二醛固定液灌流固定和合理修切以暴露移植物，并电泳１０％辣根过氧化酶（ＨＲＰ）于２ｍｍ×２ｍｍ×１．５ｍｍ大小的移植物后，光镜下可见密集的ＨＲＰ顺行标记轴突严格位于海马ＣＡ１、ＣＡ３区腔隙分子层和省回分子层，呈现出与Ｔｈｙ—１免疫组织化学显示移植内嗅皮层的轴突投射完全相同的分布模式。在电镜水平上可见组织结构保存极佳的ＨＲＰ标记末梢和非标诏树突干或树突小棘形成的突触。结果表明：ＨＲＰ电泳于固定后脑组织更易被控制于特定的区域，从而避免在体注射ＨＲＰ因易于殃及或被动扩散至相邻区域所引起的结果误导，也可以获得比免疫组化方法显示突触联系更为优良的电镜图象。
Hippocampal transplantation of rat entorhinal cortex rat brain by high concentration of glutaraldehyde fixative perfusion and reasonable trimming to expose the graft, and electrophoresis 10% horseradish peroxidase (HRP) in the size of 2mm × 2mm × 1.5mm , The densely labeled HRP paraformaldehyde-labeled axons were located in the lacunar and apical molecular layers of CA1 and CA3 in the hippocampus. The axis of Thy-1 immunohistochemistry showed that the axis of transplanted entorhinal cortex Projection cast exactly the same distribution pattern. Microscopically, HRP-labeled synapses were formed on the electron microscopic level that conserved well-organized HRP-labeled termini and non-standard OST lobes or dendrites. The results showed that HRP electrophoresis was more likely to be controlled in a specific area of brain tissue after fixation, thus avoiding the misleading results of HRP in vivo caused by easy-to-be-affected or passive diffusion to neighboring areas and also could be better than immunohistochemistry Touch contact with more excellent electron microscopy images.