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目的探讨山莨菪碱（６５４－２）对创伤性急性肺损伤（ＡＬＩ）的防止作用及其可能的机制。方法观察６５４－２对创伤性ＡＬＩ兔血浆和支气管肺泡灌洗液（ＢＡＬＦ）中肿瘤坏死因子α（ＴＮＦ－α）和白介素８（ＩＬ－８）含量的影响，同时进行组织病理学的光镜和电镜检查，并用原位杂交法检测肺和其他脏器组织中ＴＮＦ－α和ＩＬ－８的ｍＲＮＡ表达。结果致伤前、后给予６５４－２，可使创伤性ＡＬＩ兔血浆及ＢＡＬＦ中的ＴＮＦ－α、ＩＬ－８明显减少、肺组织损伤程度减轻；ＴＮＦ－αｍＲＮＡ和ＩＬ－８ｍＲＮＡ的基因表达主要定位在内脏组织中的Ｍ、ＰＭＮ和血管内皮细胞（ＶＥＣ）内，表达数量明显减少。。结论６５４－２可降低血浆及ＢＡＬＦ中ＴＮＦ－α、ＩＬ－８的含量，使ＴＮＦαｍＲＮＡ和ＩＬ－８ｍＲＮＡ的基因表达下调，从而减轻或在一定程度上防止创伤性ＡＬＩ的发生和发展
Objective To investigate the preventive effect of anisodamine (654-2) on traumatic acute lung injury (ALI) and its possible mechanism. Methods The effect of 654-2 on TNF-α and IL-8 levels in ALI rabbits and BALF was observed. Meanwhile, histopathological light microscopy And electron microscopy, and in situ hybridization detection of TNF-α and IL-8 mRNA expression in lung and other organs. Results The pretreatment and post-injury administration of 654-2 could significantly reduce the levels of TNF-α and IL-8 in the plasma and BALF of traumatic ALI rabbits and alleviate the damage of lung tissue. The gene expressions of TNF-αmRNA and IL-8mRNA were mainly localized In the visceral tissues of M, PMN and vascular endothelial cells (VEC), the expression was significantly reduced. . Conclusions 654-2 can reduce the content of TNF-α and IL-8 in plasma and BALF and down-regulate the gene expression of TNFαmRNA and IL-8mRNA, so as to reduce or to a certain extent prevent the occurrence and development of traumatic ALI