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[目的]该研究旨在研究其黄酮类化合物的基因型与环境互作分析,以在栽培和育种等方面提高大麦籽粒的黄酮类化合物含量。[方法]该研究通过高效液相色谱技术研究了种植在昆明,曲靖,保山地区大麦籽粒儿茶素,杨梅素,槲皮素,山柰酚的含量,并对这几种黄酮类化合物的基因型,环境及基因型×环境四大大麦黄酮含量的相互作用的变化进行了研究。[结果]结果表明,儿茶素和山奈酚含量的基因型方差、环境方差和G×E互作方差趋势相同,为基因型变异>环境变异>G×E互作变异,均达到极显著水平;槲皮素和总黄酮含量的基因型方差、环境方差和G×E互作方差趋势相同,为基因型变异>G×E互作变异>环境变异,均达到极显著水平;杨梅素含量的基因型方差和环境方差均达到极显著水平,G×E互作方差达显著水平;杨梅素含量的基因型变异>环境变异>G×E互作变异;总黄酮含量的基因型变异>环境变异>G×E互作变异。不同大麦品种,紫光芒大麦和宽颖大麦在曲靖,昆明,保山三个地区都具有较高的槲皮素含量,其他大麦几乎不含槲皮素,研究中发现,紫光芒大麦和宽颖大麦的籽粒颜色为紫色,而其他品种的大麦籽粒颜色均为黄色。[结论]该研究中的四种黄酮类化合物及总黄酮都是主要受遗传控制及遗传环境相互作用。紫色大麦籽粒中具有较高的槲皮素含量。
[Objective] The research aimed to study the genotype-environment interaction analysis of flavonoids in order to improve the content of flavonoids in barley grain in terms of cultivation and breeding. [Method] The contents of catechins, myricetin, quercetin and kaempferol in barley grains planted in Kunming, Qujing and Baoshan areas were studied by high performance liquid chromatography (HPLC). The contents of these flavonoid genes Type, environment and genotype × environmental changes in the content of four barley flavonoids were studied. [Result] The results showed that the genotypic variance, environmental variance and G × E interaction variability of catechins and kaempferol contents were the same, which were the genotype variation> environmental variation> G × E interaction variability, both reached the extremely significant level ; Genotype variance, environmental variance and G × E interaction variance of quercetin and total flavonoid content were the same, which were the genotype variation> G × E interaction variation> environmental variation, reached the extremely significant level; the content of myricetin Genotype variance and environmental variance reached the significant level, G × E interaction variance reached a significant level; genotype variation of myricetin content> environmental variation> G × E interaction variation; genotypic variation of total flavonoid content> environmental variation > G × E Mutual variation. Different varieties of barley, purple light barley and broad barley had higher quercetin content in Qujing, Kunming and Baoshan. Other barley had almost no quercetin. The results showed that purple barley and broad barley Of the grains are purple in color, whereas other varieties of barley grains are yellow in color. [Conclusion] The four flavonoids and flavonoids in this study are mainly affected by genetic control and genetic environment. Purple barley grain has a higher quercetin content.