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对1种抗旱性蚕豆品种“尕大豆”进行幼苗期抗旱性研究。以正常供水为对照,测定了不同胁迫时间(7 d,14 d)、不同胁迫程度(轻度LS,重度WS)下幼苗形态及生理生化指标,并对胁迫条件7 d、WS处理下的蚕豆叶片进行差异蛋白组学分析。结果表明,水分胁迫降低了“尕大豆”幼苗的地上部分和地下部分的生长,14项形态及生理指标在不同胁迫程度(LS,WS)、不同胁迫历时(7 d,14 d)下的表现各有差异。其中,在长历时胁迫下,植株地下部分(根长,侧根数)和CAT含量出现增长趋势,且达到极显著水平(p<0.01)。这正是“尕大豆”表现抗旱性的原因。对7 d、WS胁迫处理下的叶片进行双向电泳差异蛋白组学分析,经t检验发现了50个差异表达蛋白点(表达量>2或<0.5)。采用MALDI-TOF/TOF分析,30个蛋白点成功鉴定(25个下调,5个上调)。对所鉴定的蛋白质按其所参与的代谢途径和生化功能分为5类,其中调节蛋白占到所有蛋白的46.7%,属于最大一类,代谢和能量占到23.3%,功能未知仅占3.3%,为最小一类。从功能分类上,可以得出下降表达的蛋白主要和胁迫防御、代谢和能量、细胞骨架以及氧化平衡有关,而上升表达的蛋白主要参与了蛋白折叠与聚集以及光合系统。
Study on Drought Resistance of One Drought - resistant Vicia faba “Time - laden Soybean” at Seedling Stage. The seedling morphology and physiological and biochemical indexes under different stress (7 d, 14 d), different stress (mild LS, severe WS) were measured with normal water supply. Differential proteomics analysis of leaves. The results showed that the water stress reduced the growth of aerial part and underground part of “Daya” soybean seedlings. The 14 morphological and physiological indexes under different stress (LS, WS), different stress duration (7 d, 14 d) The performance of different. Among them, under long-term stress, the underground parts (root length, lateral root number) and CAT content showed an increasing tendency, reaching extremely significant level (p <0.01). This is exactly why “Time Soy ” shows the reason for drought resistance. Two-dimensional electrophoresis differential proteomic analysis of leaves under WS stress for 7 days revealed 50 differentially expressed protein spots (> 2 or <0.5) by t-test. Thirty protein spots were successfully identified (25 down-regulated, 5 up-regulated) using MALDI-TOF / TOF analysis. The identified proteins were divided into five categories according to the metabolic pathways and biochemical functions involved in them. The regulatory proteins accounted for 46.7% of all proteins, belonging to the largest category, accounting for 23.3% of the total metabolites and energy, and only accounting for 3.3% , The smallest one. From the functional classification, it can be concluded that the protein whose expression is down-regulated is mainly related to the defense against stress, metabolism and energy, cytoskeleton and oxidation balance, whereas the up-regulated protein mainly participates in protein folding and aggregation and photosynthesis system.