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目的探讨动脉粥样硬化(AS)与12/15-脂氧合酶(12/15-LOX)基因启动子区甲基化状态的关系。方法将40只SD大鼠随机分为两组,即模型组和对照组,每组20只。模型组:给予高脂+维生素D3粉剂饮食;对照组正常饮食。3个月后,处死大鼠,取其主动脉弓至腹主动脉的血管组织,用改进的半定量甲基化特异性PCR法检测血管组织中12/15-LOX基因启动子区甲基化状态。结果①经苏木精-伊红染色,显微镜下观察可见模型组大鼠的主动脉有明显的粥样斑块形成,对照组未见明显变化。②按照吸光度(A)比值把全部血管组织分为低甲基化状态和高甲基化状态,模型组大鼠的血管组织的低甲基化率显著高于对照组(P<0.01)。结论血管斑块处12/15-LOX基因启动子区的甲基化状态与AS的形成相关,在AS发病过程中可能发挥着重要的作用。
Objective To investigate the relationship between atherosclerosis (AS) and methylation status of 12/15-lipoxygenase (12/15-LOX) gene promoter region. Methods Forty SD rats were randomly divided into two groups: model group and control group, with 20 rats in each group. Model group: given high fat + vitamin D3 powder diet; control group, normal diet. After 3 months, the rats were sacrificed and the aortic arch was taken to the abdominal aorta. The methylation status of 12/15-LOX gene promoter region in vascular tissue was detected by a modified semi-quantitative methylation-specific PCR. Results ① The hematoxylin-eosin staining showed that the aorta of the model group had obvious atherosclerotic plaque formation under the microscope, but there was no obvious change in the control group. ② According to the ratio of absorbance (A), all the blood vessels were divided into hypomethylation status and hypermethylation status. The hypomethylation rate of vascular tissue in model group was significantly higher than that in control group (P <0.01). Conclusion The methylation status of 12/15-LOX gene promoter region in vascular plaque is related to the formation of AS, which may play an important role in the pathogenesis of AS.