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利用AFLP标记技术对110份来源不同的菠菜(Spinacia oleracea L.)种质进行遗传多样性分析,结果表明,筛选出的20对EcoRⅠ/MseⅠ引物组合共扩增出882条带,其中208条多态性条带,多态性比率23.6%;种质间的遗传相似系数为0.64～0.87,不同来源组群的Nei’s遗传多样性指数范围为0.1887～0.2501,总体为0.2830,Shannon信息指数范围为0.2789～0.3793,总体为0.4337。种质间基于遗传相似系数的UPGMA聚类分析、主坐标分析与组群间基于Nei’s遗传距离的聚类分析结果基本相同,与地理来源有很高的一致性。全部供试种质可分为两类,欧美、西亚、东亚及中国北方种质聚为一类,部分日本种质和中国的南方种质聚为另一类,AFLP标记能很好地从分子水平揭示菠菜资源的亲缘关系。由亲缘关系推测中国的南、北方菠菜种质可能有着不同的起源。
AFLP markers were used to analyze the genetic diversity of 110 spinach (Spinacia oleracea L.) germplasm. The results showed that 882 bands were screened from 20 pairs of EcoRⅠ / MseⅠ primer combinations, of which 208 The genetic similarity coefficient of germplasm ranged from 0.64 to 0.87. The Nei’s genetic diversity index ranged from 0.1887 to 0.2501, the overall population was 0.2830, Shannon’s information index range was 0.2789 ~ 0.3793 for a total of 0.4337. The UPGMA cluster analysis based on genetic similarity coefficient between germplasms showed that the results of cluster analysis based on Nei’s genetic distance between the two groups were basically the same and were highly consistent with the geographical origin. All the tested germplasm can be divided into two types. The germplasms of the Northern Germplasm in Europe, the United States, Western Asia, East Asia and China are clustered together, and some Japanese germplasm and the southern germplasm of China are grouped into another group. The AFLP marker can well differentiate from the molecular The level reveals the genetic relationship of spinach resources. It is inferred from the genetic relationship that China’s south and north spinach germplasm may have different origins.